相关实验视频

Updated: Aug 1, 2026

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

11.1K

在全血中对T细胞激活反应的高维基-CyTOF表征

Ilyssa E Ramos1,2,3, Teresa S Hawley2,4, Kevin Rose2

  • 1Protein and Chemistry Section (PCS), Research Technologies Branch (RTB), National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.

Bio-protocol
|October 27, 2025
PubMed
概括

这项研究引入了一种质量细胞计 (CyTOF) 协议,用于分析全血T细胞信号,克服流细胞计的局限性. 该方法可以对关键路径进行高维分析,改善临床应用的T细胞激活评估.

关键词:
结冷保存是一种冷保存.希利奥斯的CyTOF是什么意思体流动的体流动这种方法是Prot1/Thaw-Lyse.斯坦福大学的人类免疫监测核心 (HIMC)干细胞激活剂尾酒激发T细胞的刺激这是全血,全血.

更多相关视频

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

9.8K
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells08:09

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

9.8K

相关概念视频

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...