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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.5K

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相关实验视频

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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有效的circRNA检测使用渐进逆转录酶 uMRT.

Ruben Warkentin1, Anna Marie Pyle1,2,3

  • 1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.

Bio-protocol
|October 27, 2025
PubMed
概括

一种新的RT-PCR方法使用UltraMarathonRT有效检测和测序各种大小和结构的圆形RNA (circRNAs). 这种简化的协议绕过了克隆和处理,加速了RNA研究和治疗开发.

科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 生物技术是生物技术.

背景情况:

  • 循环RNAs (circRNAs) 是稳定的RNA分子,在基因调节和疾病中发挥作用.
  • 精确检测和验证circRNAs对于RNA研究和治疗应用至关重要.
  • 现有的circRNA验证方法往往耗时,缺乏吞吐量,或无法检测结构化或小型circRNAs.

研究的目的:

  • 开发一种简化,经济高效,多功能的RT-PCR协议,用于circRNA检测和测序.
  • 克服当前处理结构化,重复性或小型circRNAs的方法的局限性.
  • 为了使各种circRNA物种的精确测序,而无需复杂的样本处理.

主要方法:

  • 使用超马拉松RT (uMRT),一种高度过程的逆转录酶,用于RT-PCR.
  • 开发了一种用于直接测序RT-PCR片的协议,而无需克隆或进一步处理.
  • 应用了该方法来检测和测序各种circRNA,包括结构化,重复性和小 (<150nt) 物种.

主要成果:

  • 基于MRT的协议有效地检测和测序circRNAs,无论结构,长度或序列的复杂性.
  • 该方法使小circRNAs (<150nt) 和大,结构化的circRNAs在没有先前克隆的情况下进行测序.
关键词:
纳米孔测序的测序这是RT-PCR.滚动圈放大器 滚动圈放大器桑格尔测序是什么意思超级马拉松RTRT 的时间环RNA 环RNA 是一个环RNA.

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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

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相关实验视频

Last Updated: Jan 14, 2026

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  • 与现有的高通量方法相比,该协议显著缩短了处理时间.

    • 结论:

    • 这种新的RT-PCR协议为circRNA检测和测序提供了一种精确,高效和简化的方法.
    • 该方法广泛适用于各种circRNA类型,促进基础研究和生物技术应用.
    • 该协议能够处理多样化的circRNA结构和大小,进步RNA疗法和生物标志物发现领域.