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在小鼠大脑组织中增强RNA保存:一种将心脏输液与高盐水浸泡相结合的策略

Qinyu Ge¹, Erteng Jia², Tian Wen³

¹State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

|October 27, 2025

概括

一个新的保存策略在空间转录组学采样期间保护RNA完整性. 这种方法确保了高质量的RNA,用于在小鼠大脑研究中准确的基因表达分析.

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
基因组学就是基因组学.

背景情况:

空间转录学能够在组织内绘制基因表达映射.
对空间转录组学的毛细小针采样可能会导致RNA降解.
预分析变异性影响低密度空间转录学准确性.

研究的目的:

在基于毛细管的空间转录组学过程中开发和评估RNA完整性的保存策略.
为了最大限度地减少小鼠大脑空间奥米克的预分析变异性.
为了提高RNA质量,提高基因检测.

主要方法:

用酸盐缓冲盐水 (PBS) 进行小鼠心脏 perfusion.
在切割前将大脑组织浸泡在高盐溶液中.
在低温条件下进行微区域采样.

主要成果:

保存策略保持了RNA完整性数 (RINe) >7.5在25°C下6小时,在4°C下24小时>8.5.
与对照组相比,减少了3'转录偏差.
提高了细胞类型标记基因的检测 (p < 0.05).

结论:

开发的策略有效地保护了空间转录组学的RNA完整性.
这种方法提供了一个标准化的框架,以减少分析前的变化.
改善的RNA质量支持在小鼠大脑研究中更准确的空间奥米克分析.

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