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单原子桥 Au 纳米酶在酸性介质中增强类似葡萄糖氧化酶的活性

Xin Luo¹, Feilong Tan¹, Zhenglong Mao¹

¹National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Life and Health Sciences, Hubei University of Technology Wuhan 430068 P. R. China.

Chemical science

|October 27, 2025

概括

我们开发了新型的黄金纳米酶与铁单个原子,显著提高葡萄糖氧化酶类活性在酸性条件下. 这一进步使得新的传感器能够检测有机农药.

科学领域:

纳米材料科学科学纳米材料科学
催化剂是一种催化剂.
生物医学工程生物医学工程

背景情况:

黄金纳米酶 (Au NPs) 在模仿葡萄糖氧化酶 (GOx) 时表现有前途,但在催化活性和pH依赖性方面面临挑战.
开发高效和稳定的纳米酶用于特定的应用,如生物传感仍然是一个关键的研究领域.

研究的目的:

为了合成和表征Fe单原子桥接Au纳米酶 (Au NPs/FeNC) 具有双催化位点.
研究Au NPs/FeNC增强的葡萄糖氧化酶类活性和反应机制,特别是在酸性介质中.
为了证明Au NPs/FeNC在用于农药检测的葡萄糖级联系统中的应用.

主要方法:

合成 Fe 单个原子桥接 Au 纳米酶 (Au NPs/FeNC).
电化学和理论分析以阐明反应机制和电荷转移.
在现场监测催化中间体.
构建和测试一个便携式,基于凝的传感器系统.

主要成果:

与Au NPs/NC相比,Au NPs/FeNC在酸性介质中的GOx类活性增加了3.7倍.
从Au转移到Fe的电荷转移促进了O2吸附和协同的葡萄糖氧化.
在酸性环境中识别独特的Au-Fe-OO中间体,提高催化效率.
在pH值为4.0时实现了最佳的GOx和过氧化酶类活性.
一个基于Au NPs/FeNC的便携式传感器成功检测了有机农药.

结论:

在AU纳米酶中的Fe单原子桥接有效地增强了在酸性条件下的催化活性和稳定性.
在Au NPs/FeNC中的双催化站点和独特的中间体为设计高性能纳米酶提供了一个有希望的策略.
开发的基于纳米酶的传感器显示了环境监测和诊断中的实际应用潜力.

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