相关实验视频
Updated: Jan 14, 2026

The MPLEx Protocol for Multi-omic Analyses of Soil Samples
Published on: May 30, 2018
多组学识别了与菌失调和脂质代谢失调相关的严重喘内型
Shuang Liu1,2, Zhiwei Lin1,3, Liuyong You1
1Department of Clinical Laboratory, Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, Guangzhou Laboratory, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China.
这项研究揭示了基于气道微生物组和代谢组相互作用的独特喘亚型. 结果确定了个性化喘治疗的新生物标志物和治疗点.
科学领域:
- 呼吸系统医学 呼吸系统医学
- 微生物组研究 微生物组研究
- 代谢学 代谢学 代谢学
背景情况:
- 支气管喘是一种复杂的炎症性呼吸道疾病,异质性不明.
- 呼吸道微生物组和宿主新陈代谢在喘亚型中的作用需要进一步研究.
研究的目的:
- 研究呼吸道微生物组和代谢组在定义喘内型的整合作用.
- 确定新的诊断生物标志物和喘潜在的治疗点.
主要方法:
- 在唾液样本上利用了16S rRNA测序和非目标代谢学.
- 使用无监督的集群来识别喘亚组.
- 分析了微生物-代谢物相互作用.
主要成果:
- 在喘患者中鉴定出不同的微生物概况,其中缩了Streptococcus,Veillonella和Prevotella.
- 揭示了脂质代谢途径的失调,并确定了cis-aconitic acid和LPE 20:1作为诊断生物标志物.
- 喘分为两种类型:一种严重的链球菌占主导地位的群体,具有代谢障碍,另一种较轻的群体类似于对照群体.
结论:
- 由特定的微生物组-代谢组相互作用驱动的划定喘内型.
- 提供了新的诊断生物标志物和喘途径特定的治疗点.
- 强调了用于喘精密医学的综合多学科方法的价值.
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