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构建培酶基因工程微生物及其在烟草纸上的应用

Jiafeng Bai¹, Xiaoqin Peng², Yi Zhou¹

¹Technical Center of China Tobacco Guangxi Industrial Co. Ltd, Nanning, 530001, People's Republic of China.

Open life sciences

|October 27, 2025

概括

这项研究设计了一种酵母菌株,以产生聚甲氨酶 (PG) 酶,有效降低烟草中的点氨酸. 再组合酶显著改善了烟草纸的质量和感官属性.

科学领域:

生物技术是生物技术.
酶工程是什么? 酶工程是什么?
农业科学农业科学

背景情况:

素对香烟的感觉质量产生负面影响.
减少烟草叶中的点含量对于提高产品质量至关重要.

研究的目的:

为了设计一种酵母菌株,以产生聚甲氨酸酶 (PG) 来降解烟草中的点氨酸.
为了优化复合PG酶在烟草加工中的应用.

主要方法:

从 *Aspergillus niger* 中提取并克隆了多聚氨酸酶 (PG) 基因,并将其转化为 *Pichia pastoris*.
构造和转换的重组等离子体pPICZαA变成 *Pichia pastoris* X33.3.
净化了重组PG酶,并评估了它在烟草纸上降解点氨酸的活性.

主要成果:

成功构建了一个表达PG基因的工程酵母菌株X33/pPICZαA-PG.
再组合菌株在36小时内产生了具有高活性 (2872.91 U/mL) 的原始酶.
优化添加0.8%的原始酶减少了烟草纸质pectin的17.53% (从3.65%到3.01%),最佳的感觉效应在0.4%.

结论:

改造的酵母菌株有效地产生聚甲氨酸酶,用于烟草中的pectin降解.
再组合PG酶的应用显著改善了烟草纸中的pectin含量和感觉质量.
这种生物技术方法为提高烟草加工和产品质量提供了一种可行的方法.

关键词:

基因工程是基因工程,是基因工程.质素降解质素降解这种药物是pectinase.烟草纸的烟草纸,就是烟草.

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