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在公共蛋白质数据库中发现数百个外源和内源RNA病毒RdRp序列在未表征序列中
Katherine Brown1, Andrew Edwin Firth1
1Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
Virus evolution
|October 27, 2025
概括
研究人员选了公开的蛋白质数据库,寻找未表征的RNA病毒RNA依赖RNA聚合酶 (RdRp) 序列. 他们发现了数千种新的内源性病毒元素 (EVE) 和未标记的病毒,突出了大量未知的病毒多样性,并改善了病毒发现的负控制.
科学领域:
- 病毒学 病毒学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 公共蛋白质数据库包含数以百万计的无特征序列,其中许多源自RNA病毒.
- 这些未表征的序列包括内源性病毒元素 (EVE) 和未标记的病毒,使病毒发现复杂化.
- 未标记的RNA病毒RNA依赖RNA聚合酶 (RdRp) 序列在研究中经常被误用为负控制.
研究的目的:
- 选公共蛋白质存储库,以查找未表征的RNA病毒RdRp序列.
- 描述包括EVE在内的新型病毒序列及其潜在宿主.
- 提高病毒RdRp识别负控数据集的质量.
主要方法:
- 从NCBI的蛋白质和UniProt数据库中选了未表征的蛋白质序列.
- 确定了可能来自病毒RNARdRp的序列.
- 对已识别的序列进行了详细的表征和临时的分类学注释.
主要成果:
- 确定了3560个未表征的RNA病毒RdRp序列,其中许多代表了新的EVE.
- 发现了新的病毒群,例如线虫中的球状病毒和植物中的线粒状病毒.
- 发现了错误标记病毒序列的实例,并确定了具有RdRp相似性的非RdRp序列.
结论:
- 公共蛋白质数据库包含大量未知的病毒多样性,包括众多EVE.
- 目前对EVE多样性的理解是有限的,还有很多尚未被发现的东西.
- 提供了改善负控的建议,以加强病毒发现工作.
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