相关实验视频

Updated: Jan 14, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

多重检测和 MALDI-TOF NAMS 的应用,用于猪腹病原体

Jiangbing Shuai^1,2, Shiqi Song³, Xiao Han^2,4

¹Hangzhou Customs Technical Center, Hangzhou, China.

Microbiology spectrum

|October 27, 2025

概括

一种新的多重核酸质谱检测方法快速检测出八种主要的猪腹病原体,改善了混合感染的诊断,并加强了猪健康管理. 这种方法为疾病控制提供了灵敏和高通量解决方案.

科学领域:

兽医微生物学兽医微生物学
分子诊断学分子诊断学
质谱测量质量谱测量

背景情况:

由于复杂的病毒和细菌共感染,猪腹病对猪健康和生产构成重大挑战.
准确和快速的病原体识别对于有效的疾病控制,减少经济损失和保护公共健康至关重要,特别是考虑到动物感染潜力.
现有的诊断方法在混合感染中经常难以同时检测多种病原体.

研究的目的:

开发和验证一个多重的矩阵辅助激光溶解/电离飞行时间核酸质谱法 (MALDI-TOF NAMS) 试验,用于同时检测八种关键的猪胃肠道病原体.
与实时定量PCR (qPCR) 相比,评估开发的MALDI-TOF NAMS试验的分析性能和诊断准确性.

主要方法:

开发多重PCR和单基扩展策略,同时放大和检测目标病原体核酸.
使用MALDI-TOF质谱仪检测和潜在的延伸产品的基因型.
分析验证包括检测极限 (LoD),分析特异性和可重现性评估.
临床验证涉及测试242个样本与qPCR作为参考标准.

主要成果:

MALDI-TOF NAMS试验表现出高分析特异性,没有交叉反应性.
检测极限 (LoD) 在12.20至33.59副本/μL之间.
在批量内和批量间的可重复性证明了100%的检测率.
临床验证显示98.3%的灵敏度,99.5%的特异性和96.2%的整体一致性与qPCR.
该试验在混合感染期间检测病原体方面表现良好.

结论:

开发的MALDI-TOF NAMS试验是一种敏感,特异和高通量方法,用于同时检测主要猪腹病原体.
这种测试为诊断复杂混合感染和改善猪病监测和控制策略提供了有价值的工具.
该方法的适应性允许潜在的扩展,包括额外的病原体目标.

关键词:

马尔代夫人的名字多重检测检测多重检测猪腹病原体猪腹病原体

更多相关视频

Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach

Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach

Published on: October 7, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...