评估生物银行尿样的使用对人类进行的一些研究
Sromona D Mukherjee1, Ava Adler2, Thien Dang1
1Department of Cardiovascular and Metabolic Sciences, Cleveland Clinic, Cleveland, Ohio, USA.
Microbiology spectrum
|October 27, 2025
概括
生物银行尿样可可靠地用于临床骨干组研究. 这种验证允许进行更大规模的研究,研究尿路微生物群及其与尿道疾病的关联.
科学领域:
- 微生物学 微生物学
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 这是生物银行.
背景情况:
- 泌尿道微生物组 (urobiome) 与尿道疾病有关,但临床研究通常很小.
- 小样本大小限制了在复杂条件下区分疾病表型的能力.
- 生物储存的尿液样本为更大规模的细胞组研究提供了潜力.
研究的目的:
- 为了验证生物银行尿样的使用,用于临床医疗群体研究.
- 根据污染物,DNA完整性,细菌过度生长和性别特异性种群的保存来评估生物银行尿液质量.
- 为了比较来自生物库的微生物组数据与新收集的尿样.
主要方法:
- 对来自118名患者的174个尿样 (生物银行和新鲜) 的基因组学分析.
- 包括积极和消极控制来评估数据质量.
- 根据以下标准进行评估:污染物水平,DNA质量,细菌占主导地位和性别特异的种类.
主要成果:
- 在生物库和新鲜样本 (P < 0.001) 之间观察到多样性的显著差异 (alpha/beta).
- 这些多样性差异在很大程度上归因于研究特定的变化.
- 在使用标准化协议时,生物库样本的数据与新鲜样本的数据相当.
结论:
- 经过验证的生物库尿液样本适合临床研究.
- 这使得更大规模的尿道疾病病例控制研究成为可能.
- 生物银行扩大了对新兴药房领域的研究能力.
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