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Updated: Jan 14, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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通过一个简短的ORF编码"无稽之谈"的基因翻译,立即向上游定位,以促进基因翻译

Junyi Cao1, Sarah Goldberg1, Roee Amit1,2

  • 1Department of Biotechnology and Food Engineering, Technion─Israel Institute of Technology, Haifa 32000, Israel.

ACS synthetic biology
|October 27, 2025
PubMed
概括

研究人员使用非功能开放阅读框架 (nfORF) 开发了一种新型合成遗传组件,以显著提高细菌蛋白转化水平. 这种工程化的nfORF可以在大肠杆菌中增加基因表达的20倍.

关键词:
表达力提升 提升表达力非功能性的开放式阅读框架.核糖体伸展是指核糖体伸展.目标打开阅读框 打开阅读框翻译性合器是什么意思翻译性的重新启动.

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