使用不连续的梯度离心法净化Cryptosporidium卵泡净化
1Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention (Retired), Atlanta, GA, USA.
Methods in molecular biology (Clifton, N.J.)
|October 27, 2025
概括
本研究详细介绍了一种用于实验室研究的Cryptosporidium卵囊净化方法. 纯化的卵囊可以维持六个多月的生命力,使得它可以用于各种研究应用.
科学领域:
- 寄生虫学的寄生虫学
- 传染性疾病 传染性疾病
- 分子生物学分子生物学
背景情况:
- 囊对实验室研究至关重要.
- 卵囊可以来自受感染的动物或人类.
- 标准实验室设备可以用于净化.
研究的目的:
- 描述一种可靠的方法来净化Cryptosporidium卵.
- 概述净化卵囊的最佳储存条件.
- 突出纯化卵的适用于各种研究应用的适用性.
主要方法:
- 使用桌面离心机和微离心机进行卵囊净化.
- 存储在补充抗生素的缓冲器或二酸盐中.
- 评估卵囊活力和随时间的传染性.
主要成果:
- 净化后的卵囊在六个多月内保持活力.
- 卵巢活力在一年后显著下降.
- 对于长期隔离维护,建议在宿主中进行连续过渡.
结论:
- 介绍了一种用于卵囊净化的标准化方法.
- 纯化的卵囊适用于各种研究,包括动物研究,细胞培养和分子分析.
- 适当的储存和维护对于保持卵囊活力和传染性至关重要.
关键词:
离心机离心机离心机的使用方法化是什么? 化是什么?这就是Cryptosporidium.梯度 梯度是一种梯度.隔离 隔离 隔离 隔离卵囊:卵囊是一个卵囊.净化 净化 净化 净化叶片的溶液是什么意思糖果糖是什么 糖果糖是什么更多相关视频
05:31Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
Published on: September 14, 2019
10.2K
12:11A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Published on: July 9, 2012
20.9K
相关概念视频
Centrifugation
6.6K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
6.6K
Subcellular Fractionation
8.5K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Differential Centrifugation
Differential centrifugation is...
8.5K
