权重基因共同表达网络分析确定了与牛呼吸系统疾病相关的功能模块
Nooshin Ghahramani1, Ali Hashemi1, Bahman Panahi2
1Department of Animal Science, Division of Animal Breeding and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran.
PloS one
|October 27, 2025
概括
牛的牛呼吸道疾病 (BRD) 与特定的免疫路径和转录因子有关. 补充因子B (CFB) 基因与BRD易感性有显著的关联,可以作为抗病能力的宝贵生物标志物.
科学领域:
- 兽医免疫学 兽医免疫学
- 系统生物学 系统生物学
- 基因组学就是基因组学.
背景情况:
- 牛呼吸系统疾病 (BRD) 是一种复杂的,多因素的牛疾病.
- BRD涉及宿主免疫系统和各种病原体之间的复杂相互作用.
- 了解BRD的分子基础对于改善牛的健康至关重要.
研究的目的:
- 通过系统生物学方法确定与BRD相关的关键分子参与者和途径.
- 为BRD建立一个监管网络,并确定潜在的生物标志物.
- 调查特定基因 (如CFB) 在BRD易感性中的作用.
主要方法:
- 使用P值组合方法 (费舍尔和Invorm) 进行了对差异表达基因 (DEGs) 的元分析.
- 权重基因共同表达网络分析 (WGCNA) 用于系统生物学分析.
- 机器学习算法被用于验证,并建立了一个监管网络.
主要成果:
- 鉴定了1908个常见的元基因,揭示了BRD受影响的牛中特定的共同表达模块 (蓝色,棕色,绿色,黄色) 的显著变化.
- 在BRD中涉及的关键途径包括"沙门氏菌感染"",NOD类受体信号传递"",死细胞灭绝"",收费类受体信号传递"",TNF信号传递"",IL-17信号传递"",Apoptosis"和"流感A".
- 补充因子B (CFB) 基因被确定与BRD易感性有显著关联,其表达与临床症状相关,并调节炎症反应.
结论:
- 该研究确定了关键的转录因子 (GABPA,TCF4,ELK1,NR2C2,ARNT) 调节BRD的免疫和炎症途径.
- CFB基因的差异表达是牛群中BRD易感性和临床结果的潜在生物标志物.
- 这种综合性方法为开发BRD的诊断,治疗和遗传选择策略提供了见解.
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