一种广泛的抗真菌效应剂决定了细菌和真菌之间的相互作用
Shuangquan Yan1, Yun Zou2, Tingting Wu3
1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
PLoS pathogens
|October 27, 2025
概括
这项研究揭示了一种新的细菌武器,TseN,通过第六类分泌系统 (T6SS) 传递,该系统针对真菌核. 这一发现为对抗真菌感染提供了新的途径,并提高了对抗性菌株的药物疗效.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 病变的发生和发病.
背景情况:
- 细菌-真菌相互作用在生态系统和宿主相关环境中至关重要.
- 通过分泌分子的对抗性是已知的,但直接向真菌核输送的作用因子是不太了解的.
- 六型分泌系统 (T6SS) 是一种关键的细菌武器系统.
研究的目的:
- 为了识别和描述参与王国间对抗的新型细菌效应者.
- 阐明一种新的抗真菌效应剂的作用机制和输送.
- 探索这种效应器对抗真菌病原体的治疗潜力.
主要方法:
- 来自Acidovorax citrulli.的TseN效应物的识别和特征.
- 对TseN及其免疫蛋白TsiN的结构和生物化学分析.
- 共同培养的细菌-真菌细胞的转录组概况.
- 在活体实验中使用小鼠皮肤模型进行实验.
主要成果:
- 一种新的抗真菌核酶效应剂,T6SS传递的T6SS,被确定.
- TseN针对真菌核,抑制各种真菌物种,包括Candida auris.
- T6SS攻击诱导真菌细胞重编程,影响DNA修复和耐药性.
- TseN在体内减少真菌负担并增强醇药物的有效性方面表现出有效性.
结论:
- TseN代表了一类新的细菌效应剂,具有广泛的抗真菌活性.
- 通过T6SS介导的TseN传递为真菌的细菌对抗提供了一个新的机制.
- TseN及其同类对开发新的抗真菌疗法和克服药物耐药性的策略具有前景.
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