细胞中YB1冷震域的边际稳定性使多个核酸的结合成为可能
Puja Shrestha1,2, Sara S Ribeiro1,2, Janne Aurich2
1Lehrstuhl für Biophysikalische Chemie and Research Center Chemical Sciences and Sustainability, Research Alliance Ruhr, Ruhr University Bochum, Universitätsstraße 150, 44801, Bochum, Germany.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 27, 2025
概括
YB1冷冲击域 (CSD) 通过细胞内的C端尾部 (CSDex) 稳定. 这种通过核酸结合增强的稳定,使YB1能够结合多种联体,调解其多功能性.
科学领域:
- 分子生物学分子生物学
- 生物化学 生化学
- 结构生物学 结构生物学
背景情况:
- YB1是一种内在无序的蛋白质,具有关键冷震域 (CSD).
- YB1的多功能性和在癌症中的作用使其成为治疗点.
- 中心证券证券在体外的边际稳定性及其细胞影响是未知的.
研究的目的:
- 研究活细胞中YB1-CSD的折叠稳定性.
- 确定核酸对细胞环境中的CSD稳定性的影响.
- 了解CSD稳定性,核酸结合和YB1功能之间的关系.
主要方法:
- 在生理核酸度下体内研究了YB1-CSD折叠稳定性.
- 利用生物物理技术分析热力学和运动性质.
- 研究核酸结合对CSD稳定性的影响,区分RNA和DNA.
主要成果:
- 当CSD与它的尾部 (CSDex) 融合时,与实验室相比,CSD在细胞中表现出显著增加的稳定性 (10°C TM增加).
- CSDex存在于细胞内的折叠和展开状态的混合物中,与单独的大部分展开的CSD不同.
- 核酸结合大大提高了CSDex的稳定性,与DNA相比,对RNA观察到的效果更强.
结论:
- YB1-CSD通过其C端尾部和核酸相互作用在细胞中得到稳定.
- 在CSDex的边际,但适应性,稳定性允许它绑定多种连接体.
- 这种动态稳定机制对于YB1在细胞过程和癌症中的多方面的作用至关重要.
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