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结直肠癌发生过程中逐渐改变的基因与瘤发育免疫循环和瘤微环境有关
Bingwen Zhou1, Qingrui Liu2, Chuyue Huang2
1Jiangsu Clinical Innovation Center for Anorectal diseases of T.C.M, Nanjing Hospital of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Scientific reports
|October 28, 2025
概括
研究人员确定了血液中的关键基因,这些基因在结直肠癌发展过程中发生变化. 这些基因,特别是WARS1,对早期诊断和了解结直肠癌 (CRC) 中的免疫反应有希望.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 大肠直肠癌 (CRC) 通过腺瘤-腺癌途径发展,占大多数病例.
- 了解CRC进展过程中的分子变化对于早期诊断和有效治疗至关重要.
研究的目的:
- 在结直肠癌发生过程中识别外围血液单核细胞 (PBMCs) 的动态基因表达变化.
- 评估已识别的基因对结直肠癌 (CRC) 早期检测的诊断潜力.
主要方法:
- 从健康对照组,腺瘤和腺癌患者中对PBMCs进行转录组分析.
- 接收器操作特征 (ROC) 分析和随机森林建模用于诊断评估.
- 使用TCGA数据库进行生物信息分析,探索基因功能和关联.
主要成果:
- 基因表达概况显示,在正常-腺瘤-癌症 (N-A-C) 序列期间,免疫反应的持续增强.
- 包括WARS1在内的几种基因显示出显著的上调和有前途的诊断性能 (93.62%的准确性).
- 鉴定的基因与DNA修复,m6A调节,瘤突变负担 (TMB) 和微卫星不稳定性 (MSI) 有关.
结论:
- 在PBMCs中的特定上调基因可以作为结直肠癌 (CRC) 的潜在早期诊断标记物.
- WARS1是CRC免疫反应的可靠指标,可能会影响瘤发生和转移.
- 这项研究强调了免疫应答调节与CRC进展之间的联系.
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