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Updated: Jan 14, 2026

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通过MALDI-TOF优化丝状真菌的识别MS:关键因素的比较分析

Özlem Dogan^1,2, Busra Betul Ozmen-Capin³, John White^3,4

¹Division of Clinical Microbiology F2, Department of Laboratory Medicine, Karolinska University Hospital, Huddinge, 141 86 Stockholm, SE, Sweden.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

|October 28, 2025

概括

使用MSI-2.0数据库和板上提取的Sirius One系统实现了94.1%的准确性来识别有线状真菌. 这种方法为临床真菌学实验室提供了快速有效的工作流.

科学领域:

临床微生物学临床微生物学
菌类学菌类学是指菌类学.
质谱测量质量谱测量

背景情况:

在临床实验室中,识别状真菌是个挑战.
飞行质谱的矩阵辅助激光脱吸/电离时间 (MALDI-TOF MS) 正在发展为模具识别.

研究的目的:

评估两个Bruker MALDI-TOF MS系统 (Sirius One,Microflex 3.1) 的使用情况.
评估不同的提取协议和用于真菌识别的数据库配置.

主要方法:

分析了68种丝状真菌分离物.
使用的 in-tube 和 on-plate 蛋白质提取方法.
在Sirius One和Microflex 3.1系统上获得了光谱.
使用制造商数据库和MSI-2.0数据库.

主要成果:

西里斯一号与MSI-2数据库实现了92.6%的准确性,超过了Microflex (70.6%).
制造商数据库的结果较低 (51.5%的Sirius One,41.2%的Microflex).
使用Sirius One和MSI-2数据库进行板上提取,准确率达到了94.1%.

结论:

西里乌斯一号,MSI-2.0数据库和板上提取为真菌识别提供了有效的工作流程.
这种方法推用于临床真菌学诊断.
需要进一步优化制造商数据库.

关键词:

布鲁克生物类型状真菌是一种状真菌.马尔迪-托夫 (MALDI-TOF) 是一个国家元首.在MSI-2数据库中.这是一个微型的微软.西里乌斯一号是一号.

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