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载有奎尔塞的介酶干细胞衍生外细胞囊泡增强卵巢功能在环胺诱导的卵巢损伤

Zeynep Ece Utkan Korun^1,2, Zehra Seda Halbutogullari^3,4,5, Yusufhan Yazir^2,6,7

¹Department of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Türkiye.

Journal of ovarian research

|October 28, 2025

概括

来自沃顿的瑞丁载荷的细胞外囊泡 (EVs).

科学领域:

生殖生物学生殖生物学
干细胞疗法是一种干细胞疗法.
纳米医学是一种纳米医学.

背景情况:

过早卵巢衰竭 (POI) 对生殖健康构成重大挑战.
环胺 (CTX) 是一种常见的化疗剂,可以诱导POI.
开发用于卵巢保护和恢复的新型治疗策略至关重要.

研究的目的:

在CTX诱导POI的老鼠模型中,评估从沃顿果介质干细胞 (MSCs) 衍生出的素载荷细胞外囊 (EVs-QUE) 的疗效.
调查EVs-QUE在恢复卵巢功能方面的潜在机制.

主要方法:

从人类沃顿果MSCs中分离和表征细胞外囊泡 (EVs).
在EVs上加载Quercetin以创建EVs-QUE.
建立CTX诱导的POI大鼠模型.
卵巢内输入EVs或EVs-QUE,并通过组织学,免疫光和基因表达分析评估卵巢功能.

主要成果:

EVs-QUE治疗显著改善了卵巢形态和卵泡发育.
观察到心房毛囊减少和亡 (Casp3表达减少).
增殖 (Ki67,Pcna),抗氧化剂 (Nrf2,Sod1) 和关键生殖基因 (Star,Gdf9,Bmp15) 的升级表明卵巢功能和再生得到改善.

结论:

在POI模型中,EVs-QUE的输卵管内给药促进了卵巢恢复.
治疗效果是由抗亡,增殖和抗氧化机制调解的.
EVs-QUE为生育保护策略提供了一个有前途的途径.

关键词:

毛囊细胞生成 (Folliculogenesis) 是一种发生毛囊细胞的过程.介质细胞干细胞衍生的细胞外囊泡.氧化应激是一种氧化应激.过早的卵巢衰竭是早期的卵巢衰竭.奎尔丁 (Quercetin) 是一种青素.沃顿的果是一种果.

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