通过MdMAPK6-介导的MdWRKY9的酸化,通过与MdERF5L的相互作用来调节果的成熟
Hui Wang1, Shu-Hui Zhang1, Yu-Chen Feng1
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Horticulture research
|October 28, 2025
概括
一种新型的转录因子MdWRKY9通过与MdERF5L相互作用并抵消其抑制MdACS1.1,从而促进果的成熟. 通过MdMAPK6的酸化增强了MdWRKY9的稳定性和活性,这对果实成熟至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 水果的发育和成熟.
- 后翻译修改 后翻译修改
背景情况:
- WRKY转录因子调节植物发育过程,包括水果成熟.
- 果的成熟是一个复杂的生理过程,受基因调节的影响.
- 乙烯生物合成和翻译后修饰在水果成熟中起着至关重要的作用.
研究的目的:
- 识别和描述参与果成熟的新型转录因子.
- 阐明MdWRKY9调节水果成熟的分子机制.
- 调查MdWRKY9函数中翻译后修改的作用.
主要方法:
- 在果中识别和描述MdWRKY9.
- 在番茄中过渡和稳定的过度表达以验证功能.
- 蛋白质-蛋白质和蛋白质-DNA相互作用测试 (Y2H,BIFC,LCI,拉下).
- 液体染色学-双重质谱测量以确定酸化位点.
- 使用MdMAPK6.6进行体外酸化试验.
主要成果:
- MdWRKY9显著促进了果的成熟.
- MdWRKY9与MdERF5L相互作用,抵消其抑制乙烯生物合成基因MdACS1.1的作用.
- 在Tyr394中,MdMAPK6酸化MdWRKY9,增强其稳定性和活性.
- 由MdMAPK6进行酸化对于MdWRKY9在水果成熟中的作用至关重要.
结论:
- MdWRKY9是果成熟的关键积极调节者.
- 涉及酸化的MdMAPK6-MdWRKY9信号通路对于调节水果成熟至关重要.
- 这些发现揭示了果成熟的复杂网络中一个新的调节机制.
更多相关视频
相关概念视频
MAPK Signaling Cascades
7.9K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.9K
M-Cdk Drives Transition Into Mitosis
6.2K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.2K
Cell Signaling in Plants
6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K
PI3K/mTOR/AKT Signaling Pathway
5.3K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.3K
Master Transcription Regulators
7.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
Positive Regulator Molecules
6.5K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.5K


