Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.7K
CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
Homologous Recombination02:31

Homologous Recombination

62.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Validation of a B cell acute lymphoblastic leukemia xenograft rat model for integrated efficacy, pharmacokinetics and safety of CD19 CAR-T.

Toxicological research·2026
Same author

Targeted mutagenesis and base editing using engineered <i>Brevibacillus laterosporus</i> Cas9 with expanded target scope in rice.

Plant biotechnology (Tokyo, Japan)·2026
Same author

A novel spontaneous rat model of chronic kidney disease with mitochondrial dysfunction driven by thioredoxin insufficiency.

Translational research : the journal of laboratory and clinical medicine·2026
Same author

Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.

The New phytologist·2026
Same author

Polθ-mediated T-DNA integration is a conserved mechanism across plant species.

The Plant journal : for cell and molecular biology·2026
Same author

Efficient gene disruption with CRISPR-Cas3 in human T cells.

NAR cancer·2026

相关实验视频

Updated: Jan 13, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K

在大米中使用I-E型CRISPR-Cas3的多功能基因组编辑.

Hiroaki Saika1, Naho Hara1, Shuhei Yasumoto2,3

  • 1Division of Crop Genome Editing Research, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Japan.

Plant & cell physiology
|October 28, 2025
PubMed
概括

这项研究引入了米的新型CRISPR-Cas3基因组编辑系统,使大基因删除和基因编辑成为可能. 生态CRISPR-Cas3系统显示了高效率和可遗传的米植物突变.

关键词:
这就是CRISPR-Cas3.删除删除删除删除基因组的重新排列是基因组的重新排列.米米饭 米饭 米饭 米饭.有针对性的突变发生.

更多相关视频

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

13.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.4K

相关实验视频

Last Updated: Jan 13, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

13.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.4K

科学领域:

  • 植物生物技术 植物生物技术
  • 分子生物学分子生物学
  • 基因组编辑 基因组编辑

背景情况:

  • 来自大肠杆菌的I-E型CRISPR-Cas3 (Eco CRISPR-Cas3) 促进了哺乳动物基因组编辑中的大量删除.
  • 在植物中实施Eco CRISPR-Cas3是复杂的,因为需要7个组件同时表达.
  • 之前的植物应用仅限于玉米原塑,没有产生突变植物.

研究的目的:

  • 为米开发和验证一个Eco CRISPR-Cas3基因组编辑系统.
  • 评估在米中生成删除和基础编辑的效率.
  • 为了研究大米诱导突变的遗传.

主要方法:

  • 农细菌介导的转化被用来在米中引入Eco CRISPR-Cas3系统.
  • 用聚合酶链反应 (PCR) 和滴滴数字PCR (ddPCR) 来检测和量化基因删除.
  • 通过测序分析再生的米植物及其后代,以确认突变和遗传.

主要成果:

  • 在39-71%的转制大米中检测到删除.
  • 大量删除的等位基因频率 (高达PAM上游7.0kb) 范围为21-61%.
  • 测序证实了0.1-7.2kb的删除,包括带有插入和反转的新型等位基因,并证明了C到T基编辑.

结论:

  • 生态CRISPR-Cas3系统有效地产生大删除和米的基编辑.
  • 这一系统促进了植物的基因淘汰,删除,基因编辑和基因组重组.
  • 生态CRISPR-Cas3代表了一种有前途的工具,用于推进植物基因组编辑应用.