精子等离子体外体通过调节氧化酸化来改善羊精子在液体储存期间的运动性和线粒体功能
Tengfei Liu1, Mengmei Zhang1, Xinkang Li1
1College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, China.
Stress biology
|October 28, 2025
概括
精液等离子体外体在液体储存期间增强山羊精子运动性和线粒体功能. 这些囊泡通过改善氧化酸化和DNA完整性来保护精子.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 精子等离子体中的外体是细胞间通信的关键,影响精子特性.
- 它们在存储期间维持山羊精子运动性和线粒体功能的作用在很大程度上是未知的.
研究的目的:
- 为了研究精液等离子体对山羊精子在液体储存过程中的保护作用.
- 为了确定外体细胞是否可以改善精子运动性和线粒体功能.
主要方法:
- 隔离了山羊精液等离子体外体,并用液体储存的精子化它们.
- 使用高压结/结替换和传输电子显微镜观察外体精子-线粒体相互作用.
- 评估精子运动性,ATP含量,氧化酸化,线粒体膜潜力,ROS水平,细胞内Ca2+和线粒体DNA完整性.
主要成果:
- 液体储存降低了山羊精子的运动性,ATP含量和氧化酸化.
- 外基因组治疗显著增加了精子运动性和氧化酸化.
- 外体细胞改善了线粒体膜潜力,降低了ROS和Ca2+水平,并增强了线粒体DNA拷贝数和完整性.
- 在被外体酶处理的精子中,TFAM蛋白水平增加,这表明外体酶介导的蛋白质输送.
结论:
- 精子等离子体外体通过增强运动性和线粒体功能,在液体储存期间保护山羊精子.
- 外体细胞通过调节氧化酸化和提供诸如TFAM等有益蛋白质来改善精子质量.
- 这些发现提供了对保护精子生存能力的基于外体的策略的见解.
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