普罗比可纳诱导的丸损伤和MAPK介导的胚胎细胞的自和自
Won-Young Lee1, Ran Lee1,2, Hyeon Woo Sim2
1Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonbuk 54874, Republic of Korea.
Cells
|October 28, 2025
概括
暴露于普罗比可纳真菌杀菌剂会损害小鼠的丸,降低精子运动性和生殖细胞基因表达. 它在精子中触发了亡和自,表明了显著的生殖毒性.
科学领域:
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
背景情况:
- 普罗比可纳 (PRO) 是农业中广泛使用的三醇杀菌剂.
- 对于PRO的潜在内分泌干扰和生殖毒性存在担忧.
研究的目的:
- 研究PRO对小鼠丸功能,生殖细胞和GC-1精子的影响.
- 阐明PRO诱导的丸损伤背后的机制,包括亡和自.
主要方法:
- 小鼠接受了PRO治疗,持续了8周.
- 丸和精卵管的组织学检查.
- 对生殖细胞标记物和类固醇生成相关基因的基因表达分析.
- 对精子运动参数的评估 (VSL,LIN,STR).
- 在体外细胞毒性测定GC-1精子,包括亡和自标志物.
- 用3-甲基亚丁胺 (3-MA) 治疗,以研究自的作用.
主要成果:
- PRO暴露减少了丸直径,精卵管的大小和SALL4+细胞数量.
- 关键的生殖细胞发育基因 (Sall4,Piwil,Nanos2,Dazl) 和类固醇生成基因 (StAR,Cyp11a1,3β-HSD1) 的下调.
- 精子运动参数 (VSL,LIN,STR) 和运动精子数显著下降.
- 在GC-1精子中,PRO诱导了剂量依赖的细胞毒性,减少了增殖和增加了亡 (切割的caspase-3,BAX).
- 在GC-1精子中,PRO增加了自标志物 (LC3,ATG12,ATG5,LC3A/B).
- 与3-MA同时治疗减少了PRO诱导的自和亡标志物.
结论:
- 普罗比可纳在小鼠中诱导丸毒性,影响生殖细胞发育和类固醇生成.
- PRO会损害精子的运动性,并通过亡和自诱导精子中的细胞毒性.
- 自在PRO诱导的丸损伤中起着重要作用,可能会调解亡.
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