比较转录学揭示了致病原体特异的mTOR路径调节在感染昆虫病原菌的Monochamus alternatus中
Haoran Guan1, Jinghong He1,2, Chuanyu Zhang1
1Basic Forestry and Proteomics Research Center, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, School of Future Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Insects
|October 28, 2025
概括
控制松病需要了解真菌如何感染松树. 针对mTOR途径可以增强Beauveria bassiana的作用.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 植物病理学 植物病理学
- 分子生物学分子生物学
背景情况:
- 由Monochamus alternatus (JPS) 传播的松病 (PWD) 威胁着全球松树林.
- 昆虫病原菌Beauveria bassiana (Bb) 和Metarhizium anisopliae (Ma) 是环保的生物控制剂,但由于对宿主-病原体相互作用的理解有限,它们在现场有效性方面面临挑战.
研究的目的:
- 为了比较JPS对Bb和Ma感染的转录反应在感染后48小时.
- 阐明PWD生物控制中宿主-病原体特异性背后的分子机制.
- 确定提高真菌生物控制剂疗效的潜在目标.
主要方法:
- 双RNA测序用于分析JPS和感染真菌中的基因表达.
- 生物信息分析确定了差异表达的基因和途径.
- 在JPS中,功能验证涉及使用化学抑制剂和RNA干扰 (RNAi) 的mTOR抑制.
主要成果:
- Bb感染诱导了自和调节了JPS碳水化合物代谢,导致组织降解.
- 马氏体感染利用宿主代谢途径进行生物合成,并触发了更强的JPS免疫反应.
- 确定了mTOR信号通路作为差异性宿主反应的关键调节器.
- mTOR抑制选择性地提高了JPS中Bb诱导的死亡率,但没有影响马氏病毒性.
结论:
- 由Bb和Ma采用的不同的感染策略导致JPS的特定宿主反应.
- mTOR信号通路在调节JPS对虫病原菌的易感性方面发挥着至关重要的作用.
- 针对mTOR是一个有前途的策略,可以提高Bb作为PWD生物控制剂的现场性能.
关键词:
贝奥维里亚巴西安纳 (Beauveria bassiana) 是一个植物.甲二氧化 (Metarhizium anisopliae) 是一种含有甲的物质.这种动物是Monochamus alternatus.双重RNA-seqq 的情况.在 mTOR 路径上.松病是松病的一种疾病.更多相关视频
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