Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Overview of Exosomes01:36

Overview of Exosomes

3.5K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Molecularly Imprinted Polymers as Biomimetic Test Zones in Paper-Based Nucleic Acid Assays-Comparing Vertical and Lateral Flow Formats.

Biosensors·2026
Same author

HLA-DRB1 Allelic Combinations Differentially Shape Dendritic Cell Antigen Presentation Enhanced by Tumour Cell Line Lysate-Pulsing.

HLA·2026
Same author

Carbon nanoparticle-based lateral flow assay for the detection of specific double-tagged DNA amplicons of Paracoccidioides spp.

Mikrochimica acta·2024
Same author

Investigating the Role of Brain Natriuretic Peptide (BNP) and N-Terminal-proBNP in Thrombosis and Acute Ischemic Stroke Etiology.

International journal of molecular sciences·2024
Same author

Improved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters.

Mikrochimica acta·2024
Same author

Endotheliitis and cytokine storm as a mechanism of clot formation in COVID-19 ischemic stroke patients: A histopathologic study of retrieved clots.

Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences·2023

相关实验视频

Updated: Jan 13, 2026

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.9K

使用基于纸张的垂直流量试验对外体生物标志物进行分析.

Arnau Pallarès-Rusiñol1,2, Jennifer Marfà1,2, Rosanna Rossi1,2

  • 1Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.

Biosensors
|October 28, 2025
PubMed
概括

一个新的基于纸质的垂直流量测试 (VFA) 简化了对转移性乳腺癌研究的外体检测和分析. 这种低成本的方法提供了一个快速而可靠的替代方案复杂的技术,如流细胞计.

关键词:
性酸酶是一种性酸酶.乳腺癌 乳腺癌 乳腺癌对外群体进行分析.流动细胞计量是流动细胞计量的方法.液体活检活检液体活检基于纸张的免疫测试.垂直流量检测试验方法

更多相关视频

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.8K
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

6.6K

相关实验视频

Last Updated: Jan 13, 2026

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.9K
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.8K
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

6.6K

科学领域:

  • 生物技术是生物技术.
  • 纳米技术纳米技术
  • 癌症研究 癌症研究

背景情况:

  • 外体是纳米级的细胞外囊泡,对于细胞间通信和疾病生物标志物发现至关重要.
  • 目前的外体细胞体表征方法,如流动细胞计,往往是复杂的,昂贵的和耗时的.
  • 需要简化,易于使用的技术来检测和分析外体细胞外体,特别是在癌症研究中.

研究的目的:

  • 开发和验证一种基于纸张的垂直流量检测 (VFA),用于检测和分析转移性乳腺癌细胞系的外体.
  • 建立VFA作为一种低成本,快速和可靠的替代传统外体组分析方法.
  • 为了证明VFA在量化外体表面蛋白质方面的能力.

主要方法:

  • 该VFA使用类似ELISA的格式,具有针对外体表面蛋白 (CD9,CD63,CD81,EGFR1) 的特定抗体.
  • 与性酸酶结合的二次抗体在基质反应时产生视觉信号,在酸纤维素膜上可视化.
  • 智能手机成像被用于信号量化,测试参数被优化为性能.

主要成果:

  • 优化的VFA在不到20分钟的时间内实现了大约6 × 107外体的检测极限.
  • 使用基于珠的流细胞计的比较分析证实了一致的生物标志物表达配置文件,验证了VFA的可靠性.
  • 该试验成功检测并分析了与转移性乳腺癌相关的关键外体标志物.

结论:

  • 基于纸质的VFA提供了一种简化,低成本和快速的方法,用于外体检测和生物标志物分析.
  • 这种试验在各种研究和诊断应用中为外体细胞体表征提供了流细胞计的有希望的替代方案.
  • VFA技术有可能扩大癌症研究及其他领域对外体分析的可访问性.