在人类皮肤环境污染物代谢的高级表征
Rafael Reis1, Martine Zanini2, Guillaume Lereaux3
1Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland.
Journal of xenobiotics
|October 28, 2025
概括
超细颗粒,如甲 (BaP) 构成皮肤健康风险. 稳定同位素标记和LC-HRMS在人类皮肤模型中揭示了五种关键的BaP代谢物,推动了污染影响研究.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 分析化学 分析化学
背景情况:
- 含有多环芳 (PAH) 的超细颗粒 (UFP),如[a]烯 (BaP),是与健康问题相关的环境污染物.
- 皮肤是环境污染物的首要目标,需要对污染物代谢进行研究.
- 现有的体外模型往往缺乏代谢活性,动物试验面临道德限制.
研究的目的:
- 在人类皮肤模型中研究[a]烯 (BaP) 的代谢命运.
- 开发和验证一种用于表征BaP代谢的新型分析方法.
- 为了识别皮肤中形成的BaP代谢物.
主要方法:
- 使用3D重建的人类表皮 (RHE) 模型来确定生理相关性.
- 使用稳定同位素标记 (SIL) 与乳标记的BaP (BaP-d12) 进行精确量化.
- 应用液体染色学-高分辨率质谱学 (LC-HRMS) 结合稳定同位素过用于代谢物识别.
主要成果:
- 在RHE模型中确定了五种不同的BaPI期代谢物.
- 检测到BaP的单氧化,二氧化和子衍生物.
- 证明了SIL-LC-HRMS在综合代谢分析中的有效性.
结论:
- 结合稳定同位素标记与LC-HRMS,提供了一个强大的工具来表征人类皮肤中的BaP代谢途径.
- 鉴定到的代谢物增强了对BaP排毒和潜在不良影响的理解.
- 这种分析策略对研究皮肤中其他环境污染物的代谢具有前景.
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