指示管:一种用于监测生物库储存中的温度外观的新解决方案
Patrick J Catterson1, Tyler T Olson1, Margaret B Penno1
1The Johns Hopkins BioBank, Genetic Resources Core Facility, Department of Genetic Medicine, Johns Hopkins University School of Medicine, 600 N. Wolfe St., 1017 Blalock, Baltimore, MD 21287, USA.
Methods and protocols
|October 28, 2025
概括
新的冷指示管为冷储存提供了视觉备份. 这些具有成本效益的设备可以检测温度偏差,确保存储期间敏感生物材料的完整性.
科学领域:
- 低温生物学和样本保存科学.
背景情况:
- 低温储存对于生物材料的生存能力至关重要.
- 现有的电子监控系统可能无法检测短暂的解-重新结事件.
- 这些未被检测到的事件对样品质量和完整性构成重大风险.
研究的目的:
- 开发和验证视觉指示管作为冷储存的备用监控系统.
- 为检测温度偏差提供一个具有成本效益的解决方案.
- 为了提高冷保存协议的可靠性.
主要方法:
- 开发两种类型的指示管:一种水性染料溶液和一种以乙醇为基础的溶液,用于超低温.
- 水性染料方法使用染料固定和分散来信号解.
- 基于乙醇的方法被校准以检测特定的温度升高 (≥10°C长达≥15分钟).
主要成果:
- 水性染料管提供了部分或完全解的清晰视觉提示.
- 基于乙醇的管子成功地表明融事件达到关键稳定性值.
- 这两种方法都提供了可靠的,即时的温度突破的视觉确认.
结论:
- 冷指示管是冷储存中的电子监视器的有效,低成本的备份.
- 这些指标增强了对敏感生物材料长期保存的信心.
- 视觉信号系统提高了冷保存实践的整体安全性和可靠性.
相关概念视频
01:13Equipments Used to Measure Body Temperature
1.7K
Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
1.7K
01:14Temperature Measurement Sites
3.2K
A thermometer measures body temperature. The common sites for measuring body temperature are the oral cavity, axillary region, temporal artery, and skin surface, such as the forehead, abdomen, and axilla. True core body temperature is assessed in the rectum, tympanic membrane, pulmonary artery, esophagus, and urinary bladder.
Oral: When assessing oral temperature, the thermometer tip should be placed under the tongue in the posterior sublingual pocket. It offers accurate readings and can be...
Oral: When assessing oral temperature, the thermometer tip should be placed under the tongue in the posterior sublingual pocket. It offers accurate readings and can be...
3.2K
01:19Assessing Body Temperature - Temporal Artery
1.0K
Here is a stepwise guide to assessing the body temperature at the temporal artery using a temporal artery thermometer
Step 1: Perform hand hygiene and don a fresh pair of gloves to prevent cross-infection and ensure patient safety.
Step 2: Explain the procedure to the patient to establish trust. Clear communication establishes trust with the patient, ensures they understand what to expect, promotes cooperation, and enhances comfort during the procedure.
Step 3: Assess the patient's...
Step 1: Perform hand hygiene and don a fresh pair of gloves to prevent cross-infection and ensure patient safety.
Step 2: Explain the procedure to the patient to establish trust. Clear communication establishes trust with the patient, ensures they understand what to expect, promotes cooperation, and enhances comfort during the procedure.
Step 3: Assess the patient's...
1.0K
01:14Assessing Body Temperature - Oral
1.4K
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
1.4K
01:14Assessing Body Temperature - Axilla
1.2K
Procedural Guide for Assessing Axillary Body Temperature using a Digital Thermometer:
Step 1: Perform hand hygiene and put on clean gloves to maintain infection control and prevent cross-contamination.
Step 2: Prepare the patient by explaining the procedure to ensure understanding and cooperation. Ensure privacy, expose the axilla, and inform the patient that minimal movement is crucial for an accurate reading.
Step 3: Adjust the patient’s clothing to expose only the axilla. It minimizes...
Step 1: Perform hand hygiene and put on clean gloves to maintain infection control and prevent cross-contamination.
Step 2: Prepare the patient by explaining the procedure to ensure understanding and cooperation. Ensure privacy, expose the axilla, and inform the patient that minimal movement is crucial for an accurate reading.
Step 3: Adjust the patient’s clothing to expose only the axilla. It minimizes...
1.2K