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相关概念视频

Replicative Cell Senescence02:15

Replicative Cell Senescence

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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
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多因素成像分析作为研究细胞衰老现象型的平台.

Shatalova Rimma1, Larin Ilya1, Shevyrev Daniil1

  • 1Translational Medicine Research Center, Sirius University of Science and Technology, Federal Territory Sirius, Olympic Ave. 1, 354340 Sirius, Russia.

Journal of imaging
|October 28, 2025
PubMed
概括

这项研究引入了一个新的成像平台,以更好地识别细胞衰老,稳定细胞衰老的状态. 多模式方法克服了当前标记者的局限性,使得老化现象型的特征更准确.

科学领域:

  • 细胞生物学 细胞生物学
  • 老年学是一门学科.
  • 生物技术是生物技术.

背景情况:

  • 细胞衰老是一种复杂的稳定细胞循环停止状态,具有各种标记物,如SA-β-galactosidase和p53.
  • 现有的衰老标记在灵敏度,特异性和上下文依赖性方面存在局限性.
  • 精确地描述衰老表型对于了解衰老和与年龄相关的疾病至关重要.

研究的目的:

  • 开发和验证一个多因素成像平台,以全面描述细胞衰老表型.
  • 在多种成像方式中整合结构和功能读数.
  • 克服老化异质性带来的挑战,提高可再生性.

主要方法:

  • 扫描电子显微镜,流细胞计和高分辨率共聚焦显微镜的整合.
  • 使用了LysoTracker,MitoTracker,SA-β-gal/FACS,以及p16INK4a免疫染色.
  • 将平台应用于体外模型:复制性衰老,基因毒性压力和来自不同年龄组的原发性纤维细胞.

主要成果:

  • 多式成像框架在不同模型中成功识别了衰老现象型.
  • 营养缺乏和基因毒性压力的结合导致了最显著的器官细胞变化,与年龄相关.
  • 该平台证明了表型异质性的影响减少,并提供了可重现的多参数终点.
关键词:
细胞衰老 细胞衰老多模式成像技术多模式成像技术进行多参数分析.复制性衰老是一种复制性的衰老.压力引起的过早衰老.

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结论:

  • 开发的成像平台为表征细胞衰老提供了一个强大而可扩展的框架.
  • 它可以检测既定和新兴的衰老表型,包括药物诱导的转变.
  • 该平台适合进行比较研究,选老化剂和老年保护剂,并完善衰老状态的定义.