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Updated: Jan 13, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

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一个无变性化的协议,用于在现场可视化短核DNA序列使用挂锁探测器与滚动圆的放大

Ryoyo Ikebuchi1, Lu Xi1, Dimitra Bouri1

  • 1Molecular Tools, Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.

PloS one
|October 28, 2025
PubMed
概括

这项研究引入了一种新的方法,用于检测DNA序列,使用挂锁探头和滚动圆放大. 这种技术在人类细胞中提供了更快,更精确的基因组DNA检测.

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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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