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来自修建湿地处理石油炼油厂废水的生物薄膜的BTEX生物降解:来自化合物特定同位素分析的见解
E K Akhiladas1, Saswati Chakraborty1, Gwenaël Imfeld2
1Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India.
The Science of the total environment
|October 28, 2025
概括
建筑湿地有效地去除,,乙和 (BTEX),主要是通过生物降解. 种植的湿地增强了微生物活动,改善了炼油厂废水处理中的BTEX转化.
科学领域:
- 环境科学 环境科学
- 环境微生物学 环境微生物学
- 生物修复是一种生物修复.
背景情况:
- 建筑湿地对于处理污染了,,乙烯和烯 (BTEX) 的炼油厂废水至关重要.
- 将生物降解与物理去除过程区分开来,了解湿地设计和微生物群落对BTEX降解的影响仍然具有挑战性.
研究的目的:
- 为了研究无氧BTEX降解在建筑湿地微观世界.
- 使用双元素化合物特定同位素分析 (CSIA) 和微生物分析,将生物降解与挥发和吸收区分开来.
- 评估湿地配置 (种植与未种植,流量类型) 和微生物群落对BTEX转换的影响.
主要方法:
- 微观宇宙实验使用来自种植和未种植的水平和垂直流构造湿地的生物膜.
- 双元素化合物特定同位素分析 (CSIA) (δ13C和δ2H) 来确认生物降解.
- 微生物群落分析,以确定参与BTEX降解的关键种类.
- 量化BTEX去除途径 (生物降解,挥发,吸收).
主要成果:
- 生物降解占BTEX总去除量的约50%,乙烯和烯比和更容易降解.
- 双重CSIA证实了生物降解,特定的丰富因子 (εC, εH) 和 Λ值表明无氧降解途径.
- 种植的湿地生物膜显示出更高的微生物活性和BTEX生物降解,可能是由于根排泄物.
- 蛋白质细菌,Actinobacteriota和Desulfobacterota被确定为参与无氧BTEX转换的关键微生物类.
- 预计挥发和吸附将与生物降解一起对整体BTEX去除作出重大贡献.
结论:
- 生物降解是BTEX在建造湿地中被清除的主要途径,特别是在无氧条件下.
- 种植的建筑湿地通过刺激微生物活动增强了BTEX生物降解.
- 综合的CSIA-微生物方法对于阐明BTEX降解机制是有效的.
- 优化建筑湿地设计,考虑到植物和微生物群落的存在,可以改善碳化合物污染的废水的生物修复.
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