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在卵巢中VLDL分泌的功能MTTP和apoB支持在热应激下小毛囊的发展
Petnamnueng Dettipponpong1, Mei-Ying Sin2, Yu-Hui Chen3
1Department of Animal Science, National Chung Hsing University, Taichung, 402202, Taiwan; Department of Animal Science and Fisheries, Rajamangala University of Technology Lanna, Lampang, Thailand.
Journal of thermal biology
|October 28, 2025
概括
卵巢中的小黄色毛囊 (SYF) 分泌非常低密度脂蛋白 (VLDL),以保持细胞活力并支持毛囊发育. 热应激会破坏这个过程,影响细胞功能和活力.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 细胞应激反应的细胞应激反应
背景情况:
- 以前的研究证实了apoB和微体三糖转移蛋白 (MTTP) 在卵巢VLDL生产中的作用.
- 小黄毛囊 (SYFs) 在发育过程中和在热应激 (HS) 状态下在VLDL分泌中的功能需要进一步定义.
研究的目的:
- 调查SYFs在正常和热应激 (HS) 条件下的卵泡发育中VLDL分泌的作用.
- 确定抑制VLDL分泌对SYF功能和生存能力的影响.
主要方法:
- 在热中性 (39°C) 和热应激 (44°C) 条件下,SYF的全组织培养.
- 评估MTTP活动,apoB表达,VLDL分泌,亡标记物 (caspase 3/7),ER压力和氧化损伤.
- 使用洛米塔皮德和米波梅森,药理上抑制VLDL分泌.
主要成果:
- 在正常情况下,SYFs增加了MTTP活性,apoB表达和VLDL分泌,以及亡.
- 抑制VLDL分泌导致甘油三的积累,雌激素的产生受损,减少增殖,加速亡.
- 热应激诱导了亡,破坏了雌醇的产生和扩散,增加了氧化损伤和炎症.
- 洛米塔皮德和米波梅森加剧了HS引起的对SYF的有害影响.
结论:
- 由SYFs分泌的VLDL对于维持细胞活力和支持卵巢毛囊发育至关重要.
- 热应激会损害SYF功能,抑制VLDL分泌会使这些效应恶化.
- 这种VLDL分泌途径在细胞适应发展卵巢毛囊的压力方面发挥着至关重要的作用.
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