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在环境层面暴露于臭氧会通过转录和替代分析诱导女性生殖能力受损

Xiaomin Liang¹, Xiaoyun Wu¹, Yuchai Tian¹

¹Shanxi Key Laboratory of Coal-based Emerging Pollutant Identification and Risk Control, Research Center of Environment and Health, College of Environment and Resource, Shanxi University, Taiyuan, Shanxi 030006, PR China.

Ecotoxicology and environmental safety

|October 28, 2025

概括

臭氧 (O3) 暴露会损害女性生殖器官并破坏荷尔蒙水平. 这项研究揭示了臭氧.

科学领域:

环境健康环境健康
生殖毒理学生殖毒理学
分子生物学分子生物学

背景情况:

臭氧 (O3) 暴露与系统性影响有关,包括生殖功能障碍,但机制尚不清楚.
来自O3的氧化损伤会引发炎症和荷尔蒙失衡,影响女性生殖健康.

研究的目的:

阐明环境臭氧暴露影响女性生殖健康的分子机制.
为了确定特定的基因和途径参与臭氧诱导的子宫和卵巢功能障碍.

主要方法:

成年雌性小鼠被暴露在受控的环境臭氧度 (0.5和1.0 ppm) 中28天.
生殖组织的组织病理学检查,血清激素水平分析,转录基因分析和替代拼接分析.
使用癌症基因组图谱 (TCGA) 和定量实时PCR (qPCR) 验证的预后生存分析.

主要成果:

暴露于臭氧会对子宫和卵巢造成显著的组织病理损伤,降低关键的生殖荷尔蒙 (雌激素,孕,AMH).
转录组分析揭示了子宫中的免疫网络失调,卵巢中的类固醇激素路径发生变化.
在子宫中发现了Mapk8和Gsk3b的异常拼接,在卵巢中发现了Fshr外子跳转,影响了枢纽基因表达 (Ddit4,Il1a,Star).

结论:

臭氧暴露通过荷尔蒙失衡,免疫干扰和失调的基因表达和RNA拼接,协同增加子宫和卵巢疾病的风险.
这项研究提供了关于臭氧毒性对女性生殖健康的多方面的机制的新型实验证据.
确定了关键基因 (Ddit4,Il1a,Star) 和剪接事件,为评估与臭氧有关的生殖风险提供了潜在的生物标志物.

关键词:

另一个替代拼接.女性生殖功能障碍女性生殖功能障碍暴露于臭氧的情况.转录基因组序列的测序

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