纤维细胞生长因子21通过CYLD表达调节影响了后突触密度95和Kalirin7的局部化
Yuko Yoshida1, Ai Shinomiya2, Ken-Ichi Hanaki1
1National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, 208-0011, Japan.
Biochemical and biophysical research communications
|October 28, 2025
概括
纤维细胞生长因子21 (FGF21) 在新生小鼠中促进神经元脊柱的形成. FGF21通过Mnu-miRNA-7240-5p抑制CYLD,保持PSD95的无处不在,并增强突触连接.
科学领域:
- 神经内分泌学神经内分泌学
- 分子生物学分子生物学
- 代谢信号传递 代谢信号传递
背景情况:
- 纤维细胞生长因子21 (FGF21) 是一种内分泌激素,主要在肝脏中产生,调节能量代谢.
- 在中枢神经系统 (CNS) 中,FGF21 作为系统信号因子起作用,但其具体功能在很大程度上仍未被阐明.
- 以前的研究表明,FGF21在调节神经元外生基因和生长激素释放激素 (GHRH) 分泌中的作用在新生小鼠下丘脑中.
研究的目的:
- 为了阐明FGF21在新生小鼠下丘脑中的精确作用.
- 确定FGF21调节基因表达并影响神经元功能的分子机制.
- 为了研究FGF21信号对基因和小RNA表达在下丘脑组织的影响.
主要方法:
- 基因和小RNA表达变化的分析,由FGF21信号在新生小鼠下丘脑组织中诱导.
- 研究了涉及FGF21,Muu-miRNA-7240-5p和圆柱状质酶63二维基因酶 (CYLD) 表达的调节途径.
- 检查了对后突触密度95 (PSD95) 无化及其与Kalirin7.7的同位化的影响.
主要成果:
- 在新生小鼠中,FGF21信号调节了特定基因和小RNA表达特征.
- 发现FGF21降低了Mnu-miRNA-7240-5p的下调,导致CYLD的内质保留增加.
- 这导致抑制了CYLD表达,保留了PSD95的无处不在,并促进了PSD95/Kalirin7的局部化.
结论:
- 通过通过Mnu-miRNA-7240-5p.p.抑制CYLD的表达,FGF21维持PSD95的无处不在.
- 通过FGF21介导的途径促进PSD95和Kalirin7.7的局部化.
- 这些发现表明,FGF21在促进小鼠下丘脑中神经元脊柱形成方面发挥着至关重要的作用.
相关概念视频
Intracellular Signaling Affects Focal Adhesions
3.5K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.5K
Introduction to Fibroblasts
3.8K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.8K
Fibril-associated Collagen
3.2K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
3.2K
TGF - β Signaling Pathway
10.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.5K
Actin Filament Depolymerization
3.8K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
In F-actin, the ADF/cofilin proteins...
3.8K
Fibronectins Connect Cells with ECM
3.3K
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
3.3K


