相关实验视频

Updated: Aug 16, 2026

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Published on: January 17, 2012

查和验证来自日本 (Paralichthys olivaceus) 的加列3相互作用蛋白

Lirong Jiang¹, Baoshan Guo¹, Yiping Lin¹

¹School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.

Developmental and comparative immunology

|October 28, 2025

概括

作为一种关键的免疫蛋白质,加勒3与热冲击相关的71kDa蛋白 (HSC71) 相互作用. 这一发现揭示了Galectin3的含义.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
生物化学生物化学

背景情况:

黄素对抗病原体的天生的免疫力至关重要.
加勒3表现出多种生物活动,包括细胞激活和细胞亡调节.
加勒3与炎症反应有关,但其确切的免疫作用尚不清楚.

研究的目的:

为了识别与Galectin相互作用的蛋白质3.
阐明加勒3在免疫反应中的机制.
探索鱼类病原体信号传递和炎症级联.

主要方法:

鱼类肠上皮细胞 (PoIEC) 用加列3过度表达等离子体感染.
使用TMT液态染色学-并联质谱法 (TMT LC-MS/MS) 的蛋白质组分析.
同免疫沉,然后是液体染色学-并联质谱学 (Co-IP LC-MS/MS) 和西部斑 (Co-IP WB).

主要成果:

TMT LC-MS/MS 和 Co-IP LC-MS/MS 确定了 cathepsin B (CTSB) 和热冲击相关的 71 kDa 蛋白 (HSC71) 作为潜在的相互作用体.
联合IP WB 确认HSC71 成为 Galectin3.3 的具有约束力的合作伙伴.
在PoIEC中,加列3的过度表达得到了通过西方 blot的验证.

结论:

热冲击相关的71kDa蛋白 (HSC71) 是加勒3.3的标蛋白.
这种相互作用有助于理解galectin3在炎症反应中的作用.
这些发现有助于理解鱼类的免疫识别和炎症途径.

关键词:

在B (CTSB) 的Cathepsin 盖莱克3 是一种蛋白质.热冲击相关的 71 kDa 蛋白质 (HSC71)相互作用的蛋白质.

更多相关视频

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

相关概念视频

Rab Proteins

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...