超声波对冷保存的酸封装肝球体进行重新加热
Rui Xu1,2, Tom Brookshaw3, Eloy Erro3
1Department of Medical Physics and Biomedical Engineering, University College London, London, UK. rui.xu@ucl.ac.uk.
Scientific reports
|October 29, 2025
概括
与传统的水浴相比,超声波回暖为冷保存细胞恢复提供了更快的方法. 虽然对生存能力有轻微影响,但它对大规模组织和器官储存充满希望.
科学领域:
- 生物医学工程 生物医学工程
- 低温生物学 低温生物学
- 再生医学是一种再生医学.
背景情况:
- 有效的冷保存和大量生物体的恢复对于细胞治疗和组织/器官储存至关重要.
- 快速体积再加热方法是必不可少的,但需要进一步开发.
- 超声波回暖对细胞活力的影响在很大程度上仍未确立.
研究的目的:
- 为了比较超声波回暖与标准37°C水浴方法的疗效.
- 为了评估不同超声波功率水平对再加热速度和细胞活力的影响.
- 为了评估肝脏球状体的回暖后生存能力和细胞数量.
主要方法:
- 含有酸盐封装肝脏球体的冷细胞被冷保存.
- 在20W和100W的超声波回暖与37°C的水浴相比较.
- 在96小时的时间内测量了回暖率,回暖后活力和活性的细胞数量.
主要成果:
- 超声波回暖显著减少了回暖时间:20W (88秒) 速度快36%,100W (34秒) 速度快350%,与黄金标准 (120±5秒) 相比.
- 低功率 (20W) 的超声波回暖显示可行性仅有1%的改善,而高功率 (100W) 则相对于黄金标准而言减少了2%.
- 不同的回暖方法之间没有观察到可活细胞数量的显著差异.
结论:
- 超声波回暖是一种比常规方法更快的替代方法,用于冷回暖.
- 较低的超声波功率水平显示了在冷保存中提高细胞活力的潜力.
- 这些发现为将超声波回暖扩展到更大容量的治疗应用提供了基础.
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