相关实验视频
Updated: Jan 13, 2026

06:16
mirMachine: A One-Stop Shop for Plant miRNA Annotation
Published on: May 1, 2021
2.9K
与mRNA的MiRNA和IsomiR相互作用的新特征
Matthew Weston1, Rony Chowdhury Ripan1, Xiaoman Li2
1Department of Computer Science, University of Central Florida, Orlando, FL, USA.
Scientific reports
|October 29, 2025
概括
这项研究表明,microRNA变体 (isomiRs) 可以独立于其父微RNA与信使RNA (mRNA) 结合,影响基因调节和疾病. 同位体Rs为复杂的生物相互作用提供了新的见解.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生物信息学是一种生物信息学.
背景情况:
- 微RNAs (miRNAs) 和它们的变体 (isomiRs) 是基因表达的关键调节者,在细胞过程和疾病发病过程中发挥关键作用.
- 了解miRNA/isomiR-mRNA相互作用对于破译基因调节至关重要,但仍然是一个尚未探索的领域.
研究的目的:
- 使用编译的嵌合式读取数据,分析人类微RNA/异构RNA和信使RNA (mRNA) 之间的相互作用.
- 确定异构R特异性向的程度,并探索异构R变异的功能影响.
主要方法:
- 编译的人类模拟读取将miRNA/isomiR段与mRNA段连接起来.
- 识别和量化了microRNA/isomiR-mRNA相互作用和特征化的isomiRs.
- 分析的序列组成 (A,T,C含量) 和结合特征 (依赖种子/独立,非种子结合).
主要成果:
- 确定了1747个同位素RNA和超过500万个miRNA/isomiR-mRNA相互作用.
- 发现具有较高A/T和较低C含量的miRNA与更多的异构R和mRNA标相关.
- 发现18.9%的mRNA点仅由异构Rs结合,这表明它们具有独特的调节作用.
- 观察到功能分歧,在这种情况下,isomiRs可能会针对不同的mRNA而不是其父miRNA.
- 揭示了种子独立结合和广泛的非种子区域参与miRNA和isomiR相互作用的显著实例.
结论:
- 异构Rs表现出明显的结合模式,并且可以独立于其父miRNA准mRNA,突出显示功能分歧.
- 这项研究为miRNA/isomiR介导的基因调节的复杂性和特异性提供了新的见解.
- 这些发现强调了在理解基因调节,疾病机制和潜在的治疗策略方面考虑异构Rs的重要性.
相关概念视频
MicroRNAs
23.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
MicroRNAs
3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
RNA Interference
27.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.8K
Nucleic Acid Structure
8.4K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
DNA Structure
DNA...
8.4K
siRNA - Small Interfering RNAs
18.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.3K
Experimental RNAi
7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K

