相关实验视频

Updated: Jan 13, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

在Pediococcus pceusentosa LL-07中依赖碳源的转录组调节和外聚糖生物合成的单糖重塑

Kuan Lu^1,2, Xueya Wang³, Ying Zhou⁴

¹Guizhou Province Key Laboratory of Agricultural and Animal Products Storage and Processing, School of Liquor and Food Engineering, Guizhou University, Guiyang, Guizhou, 550025, China.

BMC microbiology

|October 29, 2025

概括

不同的碳来源通过改变基因表达改变Pediococcus pentosaceus LL-07中的外聚糖 (EPS) 生产. 这影响了EPS.

科学领域:

微生物学微生物学
生物技术是生物技术.
食品科学食品科学食品科学

背景情况:

乳酸细菌 (LAB) 产生的外聚糖 (EPS) 在工业上具有重要意义.
碳来源影响EPS的产生和组成,但Pediococcus pentosaceus的调节机制尚不清楚.

研究的目的:

研究不同碳来源如何影响P. pentosaceus LL-07中的EPS生物合成和单糖化合物组成.
专注于EPS生产的转录调节.

主要方法:

利用RNA测序 (RNA-Seq) 来分析基因表达.
进行了KEGG和基因本体学 (GO) 分析.
分析了纯化EPS的单糖化合物组成.

主要成果:

类似的EPS在葡萄糖,果糖和乳糖中的产量.
与不同的碳源观察到不同的基因表达模式.
乳糖显著上调了Leloir路径,核酸糖生物合成和EPS集群中的关键基因.
EPS单糖的组成各不相同,乳糖/葡萄糖偏好银糖,果糖偏好曼诺糖.
GEPS-1显示出改善的抗氧化剂和温度测量特性.

结论:

碳源决定了P. pentosaceus LL-07的转录变化,改变了EPS单糖的组成,抗氧化活性和热性质.
建立了糖代谢和EPS结构之间的联系.
为功能性食品和生物技术提供了通过基质工程来定制微生物多糖的策略.

关键词:

这是一种Pediococcus pentosaceus.进行比较的基因组学.外聚糖类糖化物外聚糖类糖化物文字转录学 (Transcriptomics) 是一个学科.

更多相关视频

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Published on: April 25, 2015

相关概念视频

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

Inducible Operons: lac Operon

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...

Other Glycolytic Pathways

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Global Regulatory Systems

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...