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CCNA2和CCNB3作为卵细胞成熟的早期潜在分子候选者,来自毛囊液的累积卵泡复合细胞中

Nergis Özlem Kılıç¹, Çağrı Öner², Duygu Kütük¹

¹Department of Histology and Embryology, Medical Faculty, Maltepe University, 34844 Istanbul, Turkey.

Diagnostics (Basel, Switzerland)

|October 29, 2025

概括

这项研究研究了在卵细胞成熟过程中的Cyclin-B3 (CCNB3) 和Cyclin-A2 (CCNA2) 基因和microRNA表达. 在正常情况下,CCNB3可能表示元相I (MI) 状态,而CCNA2则表明元相II (MII) 成熟.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

卵细胞成熟涉及由表观遗传基因表达变化调节的核和细胞质发育.
环素-B3 (CCNB3) 和环素-A2 (CCNA2) 参与卵细胞成熟,但它们在元相I (MI) 和元相II (MII) 中的作用需要澄清.
微RNAs (miRNAs) 在转录后调节基因表达,影响表观遗传修饰.

研究的目的:

为了检查CCNB3,CCNA2和相关的miRNA (miR-17,miR-106b,miR-190a,miR-1275) 在MI和MII卵细胞的累积细胞复合体 (COC) 中的表达.
阐明这些分子在MI到MII过渡期间在正常 (NOR) 和减少卵巢储备 (DOR) 情况下在体外受精 (IVF) 中的功能.
为了将基因和miRNA表达与卵细胞发育阶段和受精潜力相关联.

主要方法:

来自120例试管婴儿的COC细胞的分析 (每例为NOR MI,NOR MII,DOR MI,DOR MII的30个).
基因和miRNA表达的定量PCR (qPCR);蛋白质水平的免疫组织化学 (IHC).
血素和氨酸 (H&E) 染色用于COC细胞的形态分析;临床元数据的评估.

主要成果:

在MI阶段 (NOR和DOR) 的miR-17和miR-1275显著高于MII阶段 (p <0.05).
在NOR和MII中,miR-106b水平比NOR和MII中更高. 在 NOR MI 和 DOR MI 和 DOR MII 中 (p < 0.05).
在所有组中都观察到明显的形态变异;CCNB3显示出在NOR和DOR病例之间区分MI阶段的潜力.

结论:

CCNB3可以作为MI阶段区分NOR和DOR病例的生物标志物.
CCNA2表达是卵细胞成熟到MII阶段的迹象,特别是在NOR病例中.
该研究确定了目标基因 (CCNA2,CCNB3) 和相关的miRNA之间的关系,提供了关于卵细胞发育潜力的初步数据.

关键词:

细胞循环中的蛋白质.胚胎学的胚胎学这是表观遗传印记.这是一个微型RNA.卵子细胞采集 (opu)眼睛的生成眼睛的产生

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