在头部和部瘤-外部耳膜中检测具有致癌和瘤调节潜力的病毒
Kalina Shishkova1, Ivo Sirakov2, Stoyan Shishkov1
1Laboratory of Virology, Faculty of Biology, University of Sofia "St. Kl. Ohridski", 8 Dragan Tzankov Blvd., 1164 Sofia, Bulgaria.
Biomedicines
|October 29, 2025
概括
这项研究研究了保加利亚外耳状细胞癌中的病毒,发现了低风险的HPV基因型和爱斯坦-巴尔病毒 (EBV) 联合感染. 虽然它们不是确定的病因因子,但这些病毒可能会影响癌症的发展.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 皮肤病学 皮肤病学
背景情况:
- 外侧耳环的状细胞癌是一种重要的头癌.
- 状细胞癌的病因是多因素的,怀疑瘤病毒的作用.
- 之前的研究表明,各种病毒与头癌有关.
研究的目的:
- 检测高风险和低风险的人类乳头瘤病毒 (HPV),BK多瘤病毒 (BKPyV),爱斯坦-巴尔病毒 (EBV),人类细胞巨乳病毒 (HCMV) 和简单疹病毒 (HSV) 在保加利亚患者与外部耳朵状细胞癌.
- 调查研究样本中这些病毒的潜在共感染模式.
- 探索这些病毒感染在外部耳道状细胞癌的致癌过程中的可能作用.
主要方法:
- 分析了41个来自患者的活检样本 病因未被诊断的表皮细胞癌 外部耳朵.
- 利用分子生物学技术,包括常规和嵌套聚合酶链反应 (PCR).
- 进行了序列分析,用于精确的病毒识别和表征.
主要成果:
- 在任何样本中都没有检测到高风险的HPV基因型.
- 低风险HPV基因型6/11是最常见的,其次是基因型44.
- 爱斯坦-巴尔病毒 (EBV) 是最频繁检测到的疹病毒,经常与HPV 6/11一起感染.
- 简单疹病毒1 (HSV1) 在三个同HPV基因型6/11,42和43同时感染的样本中被发现.
- 在一个样本中检测到人类细胞巨型病毒 (HCMV) 与同时存在的HPV 6/11和42.
- 简单疹病毒2 (HSV2) 在任何样本中都没有检测到.
结论:
- 这是第一个在保加利亚调查HPV,BKPyV,EBV,HCMV和HSV在外耳膜状细胞癌中的研究.
- 检测到的病毒,特别是EBV和低风险HPV基因型,在并发感染中被发现,这表明在致癌过程中可能发挥作用.
- 虽然尚未证实它们是病因因子,但这些病毒可能会通过已知的机制影响外部耳朵状细胞癌的启动或调节.
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