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水牛基因组的一次性测序策略: (I) 使用第三代技术快速产生长,高质量的读数

Federica Di Maggio^1,2, Marcella Nunziato^1,2, Elvira Toscano¹

¹CEINGE-Biotecnologie Avanzate "Franco Salvatore", Via Gaetano Salvatore 486, 80145 Napoli, Italy.

Animals : an open access journal from MDPI

|October 29, 2025

概括

这项研究使用第三代测序 (TGS) 测序了五只雄性水牛基因组,揭示了对改善肉和牛奶生产的雄性特异性遗传特征的见解. 这种快速的一步基因组测序策略有助于在水牛和其他具有大型基因组的农场动物中进行遗传选择.

科学领域:

基因组学就是基因组学.
动物科学动物科学
哺乳动物遗传学哺乳动物遗传学

背景情况:

水牛 (Bubalus bubalis) 对于乳制品和肉类生产至关重要.
之前的基因组研究在很大程度上忽视了水牛Y染色体.
第三代测序 (TGS) 能够对复杂的基因组进行高级研究.

研究的目的:

为了使用TGS完全测序雄性水牛基因组.
为了深入了解男性特有的遗传特征.
开发一种快速,单步的基因组测序策略.

主要方法:

应用TGS长读测序,以获得高质量的全基因组测序.
利用了一个快速的生物信息管道.
在20-40×深度对五只雄牛基因组进行了测序.

主要成果:

为五只雄性水牛生成了高质量的全基因组序列.
为了解男性特有的遗传特征提供了基础.
证明了TGS对复杂的哺乳动物基因组的有用性.

结论:

该TGS方法提供了一个完整的战略,快速,一步的基因组测序.
这种方法适用于其他具有大型基因组的农场动物.
促进改善乳制品生产率的遗传选择和对水牛遗传变异性的研究.

关键词:

长时间阅读的阅读测序的测序是指测序的测序.第三代测序系统是第三代测序系统.水牛的基因组是什么进行全基因组测序.

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