线粒体翻译抑制触发了Rst2控制的转录重编程碳代谢在静态相细胞的裂变酵母酵母
Ying Luo1,2, Shaimaa Hassan1, Saniya Raut1
1Institute of Healthy Ageing, Department of Genetics, Evolution & Environment, University College London, London WC1E 6BT, UK.
Biomolecules
|October 29, 2025
概括
在裂变酵母中抑制线粒体转化会触发压力反应,并重新连接碳代谢,特别是在静止细胞中. 这种适应涉及转录因子Scr1和Rst2,对于在线粒体功能障碍期间管理细胞能量至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 线粒体有自己的基因组,通过蛋白质翻译对细胞能量至关重要.
- 线粒体功能障碍会影响核基因表达,特别是通过逆行反应.
- 了解细胞如何适应线粒体缺陷是细胞能量代谢的关键.
研究的目的:
- 研究使用氨基醇 (CAP) 抑制线粒体翻译对*Schizosaccharomyces pombe*转录组调节的影响.
- 在发酵和呼吸条件下,比较在增殖和静止阶段细胞中的反应.
- 阐明转录因子Scr1和Rst2在适应线粒体功能障碍方面的作用.
主要方法:
- RNA测序 (RNA-seq) 用于分析全球基因表达变化.
- 用氨基醇 (CAP) 治疗以抑制线粒体转化.
- 系统的基因相互作用屏幕,以确定关键转录因子的功能关系.
主要成果:
- 在葡萄糖介质中的静态相细胞显示了最显著的转录组对CAP的反应,类似于压力和逆行反应.
- 诱导的基因在细胞质碳代谢中得到丰富,表明从呼吸转向发酵.
- 转录因子Scr1和Rst2调节了常见的碳代谢基因,而Rst2对于基因诱导和核定位至关重要.
结论:
- 抑制的线粒体翻译会在静态相裂变酵母中诱导复杂的转录程序.
- 该程序包括压力和逆行类反应,由转录因子Scr1和Rst2.2调解.
- 细胞适应碳代谢来应对线粒体功能障碍,突出了对能量压力的协调反应.
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