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在长期暴露于甘酸盐后,大鼠脏的多基因变化
Favour Chukwubueze1, Cristian D Guiterrez Reyes1, Jesús Chávez-Reyes2
1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
长期暴露于草甘基除草剂 (GBHs) 会改变脏的N-甘和蛋白质,激活炎症途径. 雌性大鼠对这种由GBH诱导的毒性更容易受到影响.
科学领域:
- 毒理学 毒理学 毒理学
- 功能 功能 功能
- 分子生物学分子生物学
背景情况:
- 草甘基除草剂 (GBH) 被广泛使用,但它们对功能的分子影响尚未完全理解.
- 由于脏的分泌作用,脏容易受到外源生物的损伤.
- 以前的研究表明,GBHs可能会导致损伤.
研究的目的:
- 为了研究慢性GBH暴露后脏中的N-糖组和蛋白组变化.
- 为了阐明草甘诱导的损伤的分子机制.
- 为了确定 GBH 毒性的潜在性别特异性.
主要方法:
- 对暴露于GBHs的雄性和雌性大鼠脏组织的分析.
- 液体染色学-N-糖因子和蛋白质因子的并联质谱学.
- 识别改变的N-甘氨酸结构和蛋白质表达特征.
主要成果:
- 脏N-甘氨酸组成的显著变化,特别是fucosylated和sialofucosylated类型.
- 免疫信号和炎症通路的激活 (例如,中性粒细胞脱粒化,整合素信号).
- 转录调节器 (IL-6,STAT3,NFE2L2) 的升级,表明炎症和氧化应激.
- 雌性大鼠表现出更明显的变化,表明更高的易感性.
结论:
- 长期暴露于GBH会诱导脏中显著的N-葡萄糖和蛋白质变化.
- 暴露于GBH会激活免疫反应,炎症和氧化应激通路.
- 性别特定的反应差异表明女性可能更容易受到GBH毒性,可能涉及致癌过程.
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