肝细胞生长因子在早期与晚期实验性牙周炎中差异调节口腔微生物群
Ruotong Ji1, Xiaomin Zhao1, Zhen Chen1
1School of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction & Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou 510180, China.
Biology
|October 29, 2025
概括
肝细胞生长因子 (HGF) 在牙周炎早期和晚期不同影响口腔微生物群. 这一发现解释了HGF对牙周炎进展的多种影响,为疾病管理提供了洞察力.
科学领域:
- 微生物学 微生物学
- 口腔健康 口腔健康
- 分子生物学分子生物学
背景情况:
- 牙周炎是一种由微生物失生症驱动的慢性口腔疾病.
- 肝细胞生长因子 (HGF) 已表现出双重作用,缓解早期牙周炎,但恶化晚期疾病.
- 在疾病进展过程中,HGF对牙周微生物群的确切影响尚不清楚.
研究的目的:
- 为了研究HGF对牙周微生物群在牙周炎进展过程中的影响.
- 在不同的HGF水平下,将口腔微生物组的变化与宿主炎症反应和疾病标志物相关联.
主要方法:
- 在野生类型和高表达性HGF转基因小鼠中使用结诱导牙周炎.
- 对牙周组织和血清进行了炎症标志物 (IL-6,TNF-α) 和骨改造标志物 (CTXI,PINP) 的分析.
- 16S rRNA测序在不同时间点 (第0,7,28天) 采集的带上进行,以评估微生物社区结构.
主要成果:
- HGF显著改变了牙周结合物中的微生物多样性.
- 像Lactobacillus这样的特定品种在HGF-Tg小鼠中显示出相反的趋势,与疾病阶段相关.
- 诸如Sphingomonas之类的种类与炎症和骨重塑标记物相关;在后期阶段观察到富含IL-17信号通路.
结论:
- 在牙周炎的早期和后期阶段,HGF对带微生物群产生不同影响.
- 这些独特的微生物调节由HGF可能是其对牙周炎进展的不同影响的基础.
- 了解HGF-微生物群相互作用对于开发向性牙周炎治疗非常重要.
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