对Populus ussuriensis的低的转录组反应的分析
He Feng1,2, Yue Chang1,2, Runze Liu1,2
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Biology
|October 29, 2025
概括
了解Populus ussuriensis中的应激耐受性对于林业至关重要. 这项研究揭示了根部的关键分子机制,包括基因网络和转录因子,帮助树木适应低条件.
科学领域:
- 植物生物学 植物生物学
- 林业科学 林业科学
- 分子生物学分子生物学
背景情况:
- 对于植物生长至关重要,根从土壤中吸收它.
- 压力显著限制了森林树木的生产力,需要研究耐受性机制.
- 了解这些机制对于可持续林业实践至关重要.
研究的目的:
- 为了研究*Populus ussuriensis*中应力耐受性的分子机制.
- 分析表型和转录基因对低条件的反应.
- 为代谢构建一个基因调节网络 (GRN).
主要方法:
- 在不同度下对*P. ussuriensis*幼苗进行表型分析.
- 在低应激下在多个时间点进行根转录组的RNA测序 (RNA-seq).
- 使用高斯图形模型构建代谢相关的GRN.
主要成果:
- *P. ussuriensis*在气压力下增加了根生物质,抑制了芽生长.
- 转录组分析确定了8289个差异表达基因 (DEGs),涉及代谢,ROS清理,根部发育和激素信号传递.
- 443个差异表达的转录因子 (TF),包括MYB,AP2/ERF和bHLH,以及一个60节点的代谢GRN.被确定.
结论:
- 转录组数据为理解压下的 *P. ussuriensis* 根的分子适应提供了基础.
- 已确定的DEG和GRN为管理使用效率的复杂监管网络提供了洞察力.
- 这项研究有助于制定提高森林树木抵御限的策略.
更多相关视频
12:05A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa
Published on: June 27, 2025
1.1K
11:31High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
Published on: September 15, 2015
10.4K
相关概念视频
01:10Transcription
155.0K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.0K
01:23Stringent Response in E. coli
286
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
286
01:22Inorganic Nitrogen Assimilation
455
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
455
02:45Responses to Heat and Cold Stress
14.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.6K