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hiPSCGEM01:一个基因组规模的代谢模型,用于纤维细胞衍生的人类iPSCs

Anna Procopio¹, Elvira Immacolata Parrotta², Stefania Scalise¹

¹Department of Experimental and Clinical Medicine, Università degli Studi Magna Græcia, 88100 Catanzaro, Italy.

Bioengineering (Basel, Switzerland)

|October 29, 2025

概括

我们开发了hiPSCGEM01,这是人类诱导多能干细胞 (hiPSCs) 的第一个基因组规模的代谢模型. 该模型揭示了新的代谢途径,并有助于优化hiPSC培养用于再生医学应用.

科学领域:

代谢工程是代谢工程.
干细胞生物学干细胞生物学
计算生物学计算生物学

背景情况:

人类诱导的多能干细胞 (hiPSCs) 对于组织再生至关重要.
优化hiPSC代谢是提高其治疗潜力和产量的关键.
现有的代谢模型没有专门解决纤维细胞衍生的hiPSCs.

研究的目的:

开发第一个基因组规模的,针对纤维细胞衍生的hiPSCs的特定环境代谢模型 (GEM),命名为hiPSCGEM01.
为优化体外培养和应用提供hiPSC代谢的系统级理解.
在hiPSCs中识别新的代谢途径和必要的基因/代谢物.

主要方法:

使用基因表达综合 (GEO) 数据和RECON 3D框架构建hiPSCGEM01.
细胞外和细胞内代谢物交换和生长介质要求的整合.
应用淘汰赛模拟和流量平衡分析 (FBA) 来识别重要的代谢成分.

主要成果:

开发hiPSCGEM01,一种专门用于纤维细胞衍生hiPSCs的GEM.
通过FBA和淘汰赛模拟来识别基本基因和代谢物.
发现酸盐和异生物代谢在hiPSC适应中的意想不到参与.

结论:

hiPSCGEM01提供了一个强大的平台,用于对hiPSC代谢的in silico分析.
该模型有助于合理优化培养媒介和预测hiPSC行为.
这项工作促进了对hiPSCs在再生医学和临床结果中的理解和应用.

关键词:

重要代谢物基本代谢物流量平衡分析流量平衡分析基因组规模的代谢模型.诱导多能干细胞的诱导干细胞.

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