压力颗粒的节奏动力学在野生类型和Bmal1纤维细胞中缺乏功能性的正规循环时钟
Melisa Malcolm1,2, Julio M Pusterla1,2,3, Laura G Penazzi1,2
1Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Cordoba X5000HUA, Argentina.
International journal of molecular sciences
|October 29, 2025
概括
细胞应激颗粒 (SGs) 呈现出每日节奏,即使没有核心分子钟 (BMAL1) 也存在. 这表明替代机制调节了细胞应激反应的时间.
科学领域:
- 细胞生物学 细胞生物学
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- 循环节律控制着24小时的生物过程.
- 压力反应显示昼夜调节,但压力颗粒 (SG) 的节律调节是不太了解的.
- SGs是细胞质RNA蛋白凝结物,对于细胞应激反应至关重要.
研究的目的:
- 为了研究NIH/3T3细胞中酸诱导的压力颗粒 (SG) 的昼夜动态.
- 确定SG节律性对正规分子昼夜时钟 (BMAL1) 的依赖.
主要方法:
- 培养的NIH/3T3和Bmal1-/-小鼠胚胎纤维细胞 (MEF) 用酸进行治疗.
- 量化SG数,eIF3信号强度,以及随时间变化的区域.
- 评估了与SG相关的RNA结合蛋白和eIF2α酸化的mRNA和蛋白质水平.
主要成果:
- SG数量,eIF3强度和区域表现出~24小时的振荡.
- 这些SG节奏在Bmal1-/-MEF中持续存在,尽管振幅和阶段发生了变化.
- 观察到与SG相关的RNA结合蛋白的时间依赖性变化;没有检测到酸化eIF2α的时间变化.
结论:
- 压力颗粒的节律性可以独立于正规的BMAL1-依赖的分子钟.
- 其他振荡机制可能有助于细胞应激反应的时间调节.
- 了解SG节奏为细胞压力管理和疾病关联提供了洞察力.
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