相关实验视频

Updated: Jan 13, 2026

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

使用RNA向CRISPR/Cas13击败FRMD4A,这是与大脑发育障碍相关的因素,也是阿尔茨海默病的危险因素,揭示了它在细胞形态发生中的作用

Asahi Honjo^1,2, Hideji Yako^1,2, Yuki Miyamoto^1,2,3

¹Laboratory of Molecular Neuroscience and Neurology, Tokyo University of Pharmacy and Life Sciences, Horinouchi, Hachioji 192-0982, Tokyo, Japan.

International journal of molecular sciences

|October 29, 2025

概括

FRMD4A和FRMD4B蛋白对于神经元过程延长至关重要. 黄素hesperetin通过激活MAPK/ERK信号通路来恢复正常的神经元发育,为大脑发育障碍提供潜在的治疗益处.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

FRMD4A中的基因突变与大脑发育障碍和阿尔茨海默病有关.
FRMD4A和FRMD4B是FERM域蛋白质,可能参与中枢神经系统的细胞形态发生和极化.
在神经元形态发生过程中,FRMD4A和FRMD4B的确切作用在很大程度上仍未被描述.

研究的目的:

研究FRMD4A和FRMD4B在神经元过程延长中的功能.
探索hesperetin在恢复FRMD4A/FRMD4B受损时恢复正常神经元形态方面的潜力.

主要方法:

在初级皮质神经元和N1E-115神经元细胞系中使用CRISPR-Cas13技术对Frmd4a和Frmd4b进行 Knockdown.
评估神经元过程延长和标记物表达.
用hesperetin治疗以评估其对神经元形态和MAPK/ERK信号传递的影响.

主要成果:

击倒Frmd4a或Frmd4b显著降低了神经元细胞中的过程延长.
赫斯佩雷丁治疗挽救了由Frmd4a/Frmd4b knockdown引起的过程延长的减少.
赫斯佩雷丁刺激了MAPK/ERK酸化,这表明它在促进神经元过程中的作用.

结论:

FRMD4A和FRMD4B在调节神经元过程延长方面发挥着至关重要的作用.
赫斯佩雷丁通过调节MAPK/ERK信号通路,显示出恢复正常神经元表型的潜力.
这些发现突出了FRMD4A/FRMD4B和hesperetin作为治疗神经发育障碍的潜在目标.

关键词:

这就是FRMD4A.FRMD4B FRMD4B FRMD4B FRMD4B FRMD4B FRMD4B FRMD4B FRMD4B FRMD4B FRMD4B 没有希望,没有希望.形态发生 (morphogenesis) 是一种形态的产生.神经元细胞的神经元细胞

更多相关视频

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Published on: June 9, 2018

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...