相关实验视频

Updated: Jan 13, 2026

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

对毒性的反应:通过富硫分子和氧化预防系统调解的防御

Luis Fernando García-Ortega¹, Iliana Noemí Quiroz-Serrano², Jesús Guzmán-Moreno²

¹Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Irapuato 36824, Mexico.

International journal of molecular sciences

|October 29, 2025

概括

(II) 离子会增加细菌的突变率,特别是在细菌细菌中. 防御机制,如金属挤出和硫醇清理,有助于细菌抵消的作用.

科学领域:

环境微生物学环境微生物学
细菌遗传学细菌遗传学
毒理学毒理学毒理学

背景情况:

(II) 离子 (Hg(II)) 诱导反应性氧物种 (ROS),在真核生物中引起细胞毒性和基因毒性.
这些效应在细菌中不太清楚,因此需要在 prokaryotic 模型中进行研究.

研究的目的:

评估Hg的细胞毒性和基因毒性影响 (II) 在* Bacillus subtilis*.
分析B. subtilis对Hg (II) 暴露的总体转录反应.
调查SOS,一般压力和抗氧化剂反应在抗性中所起的作用.

主要方法:

野生类型和突变菌株 * Bacillus subtilis * 对HgCl₂的暴露.
测量突变频率对利芬素耐药性的测量 (Rif).
对*rpoB*基因突变的分析.
用RNA测序 (RNA-seq) 来确定全球的转录变化.

主要成果:

HgCl₂暴露显著增加了B. subtilis*菌株的突变频率.
*rpoB*基因的突变表明,像8-oxo-guanines这样的氧化DNA基的形成.
RNA-seq检测显示了金属排放/减少基因和硫分子合成基因的上调.
观察到铁代谢和细胞外压力基因的下调.

结论:

细菌对Hg(II) 的防御机制包括金属挤出和基于的基清理.
这些机制抵消来自破坏铁代谢的ROS诱导的损害.
应激反应途径在减轻细菌中Hg的基因毒性作用方面发挥着作用.

关键词:

这种细菌是 Bacillus subtilis.在 RNA 序列中,RNA 序列毒性的毒性在中毒性突变发生的突变发生.氧化应激是一种氧化应激.富含硫的分子中的分子.

更多相关视频

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

相关概念视频

Sulfur Assimilation

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...

Other Stress Responses in Bacteria

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

Microbial Nutrition

Microbial Nutrition

Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.