在羊水中对抗真菌药物的Candida敏感性:初步研究
Silvia Gabriela Ionescu1, Cristina Daniela Dimitriu2,3, Demetra Gabriela Socolov3,4
1Department of Preventive Medicine and Interdisciplinarity (IX)-Microbiology, Grigore T. Popa University of Medicine and Pharmacy Iași, 700115 Iași, Romania.
Pathogens (Basel, Switzerland)
|October 29, 2025
概括
这项研究评估了来自羊水的Candida物种的抗真菌耐药性. 一些Candida菌株表现出对可纳和菌素的耐药性,凸显了需要进行物种级抗真菌敏感性测试的需要.
科学领域:
- 医学真菌学 医学真菌学
- 产科和妇科 产科和妇科
- 传染性疾病 传染性疾病
背景情况:
- 羊水的真菌感染,特别是Candida物种,很少见,但与早产和新生儿不良后果有关.
- 评估抗真菌敏感性对于管理这些感染至关重要.
研究的目的:
- 评估从羊水中分离出来的Candida物种的抗真菌易感性模式.
- 为了确定对常见抗真菌剂耐药性的流行率.
主要方法:
- 使用Sabouraud agar,VITEK® 2 Compact系统和实时PCR (RT-PCR) 来分析来自羊水的40个探索性Candida分离物.
- 基于最小抑制度 (MIC) 的抗真菌敏感性测试进行.
主要成果:
- 最常见的物种是Candida albicans (67.5%),其次是Pichia kudriavzevii,Nakaseomyces glabratus,Candida parapsilosis和Candida dubliniensis. 这些物种是最常见的物种.
- 两种C. albicans分离体表现出对可纳的耐药性,所有C. albicans分离体对米卡金具有耐药性.
- 氨基氨酸B证明了对所有测试的隔离物的有效性. 通过RT-PCR检测证实了C. albicans的DNA存在.
结论:
- 在Candida菌株中出现抗真菌耐药性的出现,需要在物种层面进行鉴定和敏感性测试.
- 精确的物种识别有助于临床医生优化抗真菌管理,改善患者的治疗结果.
相关概念视频
01:27Cryptococcal Meningitis
Cryptococcal meningitis is a life-threatening opportunistic infection predominantly associated with HIV/AIDS, accounting for over 100,000 deaths annually worldwide. However, it also affects individuals with other forms of immunosuppression, including those undergoing immunosuppressive therapy, organ transplant recipients, patients with innate immunodeficiencies, and individuals with hematological disorders. The infection is caused mainly by Cryptococcus neoformans and Cryptococcus gattii,...
01:20Candidiasis
Candidiasis is a fungal infection caused by opportunistic species of Candida. It can affect various anatomical sites, including the skin, oral cavity, nails, and genitourinary tract. Among its forms, vaginal candidiasis is the most common type of mucosal infection. It typically results from the overgrowth of Candida albicans in the vaginal mucosa. Under normal conditions, C. albicans exists as a commensal organism within the vaginal microbiota, regulated by the dominance of lactobacilli, which...
01:15Antifungal Agents
Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...