定数感应的循环di-GMP调节及其对Sinorhizobium fredii中的第六类分泌系统功能的影响
Juan Aranda-Pérez1, María Del Carmen Sánchez-Aguilar1, Ana María Cutiño-Gobea1
1Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes 6, CP41012 Sevilla, Spain.
Microorganisms
|October 29, 2025
概括
这项研究表明,循环二-GMP (c-di-GMP) 和定数感应 (QS) 信号直接调节Sinorhizobium fredii.中的第六类分泌系统 (T6SS). 这一发现澄清了管理有益的树根菌-植物共生的分子机制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 豆-豆共生涉及复杂的分子信号通路.
- 六型分泌系统 (T6SS) 在细菌竞争和宿主相互作用中发挥作用,但其在共生中的功能尚不清楚.
- 像N-acyl homoserine lactones (AHLs) 和循环di-GMP (c-di-GMP) 这样的定数感应 (QS) 信号调节细菌行为,包括生物膜形成和外聚糖类生产,但它们对分泌系统的联合作用尚未被探索.
研究的目的:
- 为了研究c-di-GMP,QS和T6SS在Sinorhizobium fredii中的表达之间的相互作用.
- 了解有利的树枝生物与植物相互作用背后的调节机制.
主要方法:
- 过度表达异构的迪加尼酸环酶,以增加三种Sinorhizobium fredii菌株的细胞内c-di-GMP水平.
- 对生物膜发展的评估.
- β-银酸酶记者测定测量在不同的c-di-GMP和AHL度下测量T6SS转录激活.
主要成果:
- 较高的c-di-GMP水平导致了对生物膜形成的菌株特异性影响,在两个菌株中增强了生物膜,在一个菌株中减少了生物膜.
- 两种高c-di-GMP和/或AHL度都被证明可以转录激活T6SS.
- 在c-di-GMP,QS信号和T6SS表达之间建立了直接的监管联系.
结论:
- c-di-GMP和QS信号通路直接参与Sinorhizobium fredii中T6SS的调节.
- 这些发现阐明了一种多层次的控制系统,影响着根茎植物共生.
- 这项研究强调了对c-di-GMP的菌株特定反应,强调了在共生相互作用中细菌社会行为的复杂性.
关键词:
N-乙类同类素乳 (AHLs) 的使用.辛诺希比乌姆弗雷迪 (Sinorhizobium fredii) 是一个有机物.第六类分泌系统 (T6SS)生物膜是一种生物膜.循环的二-GMP (c-di-GMP) 的时间.豆类 豆类 豆类 豆类结节是指结节的形成.多数感应 (QS) 是指通过多数感应 (QS)同生共生是一种共生.更多相关视频
12:11Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
Published on: March 29, 2015
12.1K
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
14.6K
相关概念视频
01:28Gene Regulation in Microbial Communities: Quorum Sensing
506
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
506
01:30Bacterial Signaling
40.0K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
40.0K
01:28Global Regulatory Systems
593
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
593
01:09GPCRs Regulate Adenylyl Cylase Activity
7.3K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
7.3K
01:23Stringent Response in E. coli
286
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
286
01:17Gene Regulation During Sporulation
436
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
436