细菌感染及其细胞壁连接体不同调节三阴性乳腺癌细胞中的多克索鲁比辛敏感性
Sima Kianpour Rad1,2, Runhao Li1,2, Kenny K L Yeo1,2
1Solid Tumour Group, Basil Hetzel Institute for Translational Health Research, The Queen Elizabeth Hospital, Central Adelaide Local Health Network, Woodville South, Adelaide, SA 5011, Australia.
Microorganisms
|October 29, 2025
概括
细菌感染及其成分可以增强多克索鲁比 (DOX) 的吸收和有效性,在三阴性乳腺癌 (TNBC) 细胞. 这表明宿主-微生物相互作用影响化疗反应,突出潜在的治疗途径.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
背景情况:
- 三阴性乳腺癌 (TNBC) 是具有不良结果的侵略性.
- 瘤微生物组可能会影响TNBC的进展和治疗反应.
研究的目的:
- 研究 Pseudomonas aeruginosa 和 Staphylococcus aureus 以及它们的细胞壁组成部分 (LPS,LTA) 如何影响TNBC细胞中的多克索鲁比 (DOX) 疗效.
- 评估TNBC细胞系中的细菌吸收,持久性和对药物反应的影响.
主要方法:
- 利用 gentamicin 保护试验量化 TNBC 细胞中的细菌吸收和存活率.
- 采用流细胞计和CFU计数来分析细菌存在和对DOX反应的影响.
- 测试了细菌感染和纯化的LPS/LTA对DOX积累和细胞毒性的影响.
主要成果:
- 无论是P. aeruginosa还是S. aureus都被内化并在TNBC细胞中持续存在.
- 细菌感染和LPS/LTA显著增加了MDA-MB-468和MDA-MB-231细胞中的DOX积累和细胞毒性.
- 收费类受体 (TLR2和TLR4) 信号与调节药物吸收有关,正如LPS/LTA效应所暗示的那样.
结论:
- 细菌的存在和成分增强特定TNBC细胞系的多克索鲁比吸收和细胞毒性.
- TLR信号通路参与调解这些效应.
- 宿主-微生物相互作用对于确定TNBC中化疗反应至关重要,因此需要进一步研究.
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