化合物对感染PRRSV的猪膜巨细胞中的线粒体功能的差异影响
Abigail Williams1, Christina Bourne1, John Byrne1
1Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
Viruses
|October 29, 2025
概括
补充剂在体外没有抑制猪生殖和呼吸系统综合征病毒 (PRRSV). 然而,DL-selenomethionine改善了猪免疫细胞中的线粒体功能,支持其潜在的免疫调节作用.
科学领域:
- 动物科学动物科学
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- (Se) 是一种重要的微量矿物质,以其抗氧化和抗炎作用而闻名.
- 缺少Se可以导致氧化应激增加和免疫抑制,影响动物健康.
- 在猪中,Se补充与增强的免疫力和生长有关,具有对病毒感染的潜在保护作用.
研究的目的:
- 研究不同有机和无机化合物对猪生殖和呼吸系统综合征病毒 (PRRSV) 感染的体外影响.
- 评估对免疫细胞功能,氧化应激和感染PRRSV的猪巨细胞中的病毒载荷的影响.
- 探索作为一种替代方法来减轻猪中PRRSV诱导的挑战的潜力.
主要方法:
- 在存在或缺少各种化合物 (DL-氨酸,L-氨酸,酵母,氨酸) 的情况下,猪膜巨被分离并感染PRRSV-2.
- 进行了测试,以评估线粒体功能 (ATP生产),基因表达,氧化应激标志物和病毒载荷.
- 进行了转录组分析,以确定感染后和治疗后的基因表达变化.
主要成果:
- DL-selenomethionine显示增强了糖分和线粒体ATP的产生,表明线粒体功能得到改善.
- 在实验室中没有观察到任何测试的化合物对PRRSV具有显著的抗病毒活性.
- 转录组分析显示感染诱导的基因调节,但并没有显著改变这些变化.
结论:
- ,特别是DL-selenomethionine,在猪免疫细胞中增强了线粒体功能,但在体外没有对PRRSV表现出直接的抗病毒作用.
- 这些发现为的免疫调节潜力提供了进一步的洞察力,与之前对增强免疫力的体内观测相一致.
- 进一步的研究可能会探索在支持对猪病毒病原体的宿主防御机制中的作用.
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